Department of Radiation Oncology, Cancer Biology Division, Washington University in St Louis School of Medicine, St. Louis, MO 63108, USA.
Pathology Service, University Hospital of Salamanca, Salamanca, Spain.
Exp Cell Res. 2014 Mar 10;322(1):1-11. doi: 10.1016/j.yexcr.2013.12.017. Epub 2013 Dec 28.
In tissue engineering techniques, three-dimensional scaffolds are needed to adjust and guide cell growth and to allow tissue regeneration. The scaffold must be biocompatible, biodegradable and must benefit the interactions between cells and biomaterial. Some natural biomaterials such as fibrin provide a structure similar to the native extracellular matrix containing the cells. Fibrin was first used as a sealant based on pools of commercial fibrinogen. However, the high risk of viral transmission of these pools led to the development of techniques of viral inactivation and elimination and the use of autologous fibrins. In recent decades, fibrin has been used as a release system and three-dimensional scaffold for cell culture. Fibrin scaffolds have been widely used for the culture of different types of cells, and have found several applications in tissue engineering. The structure and development of scaffolds is a key point for cell culture because scaffolds of autologous fibrin offer an important alternative due to their low fibrinogen concentrations, which are more suitable for cell growth. With this review our aim is to follow methods of development, analyze the commercial and autologous fibrins available and assess the possible applications of cell culture in tissue engineering in these three-dimensional structures.
在组织工程技术中，需要三维支架来调节和引导细胞生长，并促进组织再生。支架必须具有生物相容性、可生物降解性，并能促进细胞与生物材料的相互作用。一些天然生物材料，如纤维蛋白，提供了一种类似于含有细胞的天然细胞外基质的结构。纤维蛋白最初是作为商业纤维蛋白原池的密封剂使用的。然而，这些池中的高病毒传播风险导致了病毒灭活和消除技术的发展，并使用了自体纤维蛋白。近几十年来，纤维蛋白已被用作细胞培养的释放系统和三维支架。纤维蛋白支架已被广泛用于培养不同类型的细胞，并在组织工程中找到了多种应用。支架的结构和发展是细胞培养的一个关键点，因为自体纤维蛋白的支架由于其较低的纤维蛋白原浓度，为细胞生长提供了一个重要的选择。通过这篇综述，我们的目的是跟踪开发方法，分析现有的商业和自体纤维蛋白，并评估这些三维结构中细胞培养在组织工程中的可能应用。
