North Carolina State University, College of Veterinary Medicine, Raleigh, North Carolina, United States of America.
North Carolina State University, Department of Statistics, Raleigh, North Carolina, United States of America.
PLoS One. 2018 Aug 28;13(8):e0202194. doi: 10.1371/journal.pone.0202194. eCollection 2018.
A group of injured yellow-bellied sliders (Trachemys scripta) and river cooters (Pseudemys concinna) were evaluated for a variety of health values at presentation to the NC State Turtle Rescue Team and prior to release. An i-STAT Portable Clinical Analyzer and CG8+ cartridges were used to determine venous blood gas and biochemical values, the packed cell volume (PCV) and total protein were evaluated using hematocrit tubes and high speed centrifugation, and a differential WBC percentage was determined manually with Diff-Quick stained blood smear slides. Forty-six turtles were sampled on presentation and twenty-three of those were sampled again prior to release. Blood values were analyzed for significant differences between samples collected at presentation and prior to release, as well as differences between surviving and non-surviving turtles. Five variables were identified as significantly different between presenting and recuperated samples: pH, pCO2, Glu, % heterophils, and % eosinophils. When comparing samples between turtles that survived versus those that did not, two variables were identified as being significant prognostic indicators; lactate and PCV. Identification of these significant variables can aid in determining patient prognosis and triage therapy for injured aquatic turtles.
一组受伤的黄腹滑龟（Trachemys scripta）和红耳龟（Pseudemys concinna）在被送到北卡罗来纳州海龟救援队接受评估时，以及在被放生之前，对其进行了各种健康指标的评估。使用 i-STAT 便携式临床分析仪和 CG8+试剂盒来确定静脉血气和生化值，使用血细胞比容管和高速离心来评估红细胞压积（PCV）和总蛋白，使用 Diff-Quick 染色血涂片手动确定白细胞分类百分比。在就诊时对 46 只海龟进行了采样，其中 23 只在放生前再次进行了采样。对就诊时和放生前采集的样本之间的血液值进行了分析，比较了存活和非存活海龟之间的差异。有五个变量在就诊和恢复样本之间存在显著差异：pH 值、pCO2、Glu、异嗜性白细胞百分比和嗜酸性粒细胞百分比。在比较存活的海龟和未存活的海龟之间的样本时，有两个变量被确定为具有显著预后意义的指标；乳酸和 PCV。这些显著变量的确定可以帮助确定患病水生海龟的预后和分诊治疗。
