The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory for Oral Biomedical Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan, China.
Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, China.
J Dent. 2018 Nov;78:83-90. doi: 10.1016/j.jdent.2018.08.012. Epub 2018 Aug 25.
This work aims to explore the feasibility of chlorhexidine-encapsulated mesoporous silica (CHX@pMSN) as a modifier of a commercial dental adhesive via the evaluation of physicochemical properties and antibacterial capabilities of adhesive-dentin interface.
Therapeutic adhesives were developed in the present study by incorporating CHX@pMSN into a commercial adhesive at four mass fractions (0, 1, 5 and 10 wt.%). The antibacterial capability on Streptococcus mutans (S. mutans) biofilm, conversion degree, adhesive morphology, microtensile bond strength (MTBS) and nanoleakage expression were evaluated comprehensively.
MTT and CLSM evaluation showed that CHX@pMSN-doped adhesive inhibits S. mutans biofilm growth, while CHX is released from the modified adhesive continuously. The incorporation of CHX@pMSN did not affect immediate bond strength at the concentration of 1% and 5% (P > 0.05). Moreover, these bonds were mainly preserved in 5% CHX@pMSN group after one month of collagenase ageing. Meanwhile, CHX@pMSN-doped adhesive groups exhibited similar nanoleakage distribution compared with the control.
This study showed that the 5% CHX@pMSN-modified adhesive achieved balance amongst unaffected immediate bonding strength, well-preserved bonds against collagenase ageing and effective inhibition of S. mutans biofilm growth.
CHX@pMSN-modified dentin adhesive can potentially extend the service life of adhesive restoration in clinic.
本研究旨在通过评估牙本质粘结界面的理化性能和抗菌性能，探索将载氯己定介孔硅（CHX@pMSN）作为商业牙科粘结剂改性剂的可行性。
本研究通过将 CHX@pMSN 掺入商业粘结剂中，制备了四种质量分数（0、1、5 和 10wt.%）的治疗性粘结剂。综合评估了对变异链球菌（S. mutans）生物膜的抗菌能力、转化率、粘结剂形态、微拉伸粘结强度（MTBS）和纳米渗漏表达。
MTT 和 CLSM 评价表明，CHX@pMSN 掺杂的粘结剂抑制 S. mutans 生物膜的生长，同时 CHX 从改性粘结剂中持续释放。在 1%和 5%的浓度下，CHX@pMSN 的掺入并不影响即刻粘结强度（P>0.05）。此外，在胶原酶老化一个月后，这些粘结在 5%CHX@pMSN 组中主要得到保留。同时，与对照组相比，CHX@pMSN 掺杂的粘结剂组表现出相似的纳米渗漏分布。
本研究表明，5%CHX@pMSN 改性粘结剂在不影响即刻粘结强度、保持胶原酶老化粘结的完整性和有效抑制 S. mutans 生物膜生长之间取得了平衡。
CHX@pMSN 改性牙本质粘结剂有望延长临床粘结修复的使用寿命。
