The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory for Oral Biomedical Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan, People's Republic of China.
Sci Rep. 2017 Aug 15;7(1):8189. doi: 10.1038/s41598-017-08633-3.
Almost half of dental restorations have failed in less than 10 years, and approximately 60% of practice time has been consumed to replace these dental restorations. As such, contemporary dentin adhesives should be modified to treat secondary caries and prevent the degradation of adhesive-dentin interfaces. To achieve this goal, we developed a versatile therapeutic adhesive in the present study by incorporating quercetin, which is a naturally derived plant extract, into a commercial adhesive at three concentrations (100, 500 and 1000 µg/mL). An unmodified adhesive served as a control. The antibacterial ability on Streptococcus mutans biofilm, conversion degree, microtensile bond strength, failure modes, in situ zymography, nanoleakage expression and cytotoxicity of quercetin-doped adhesive were comprehensively evaluated. Results showed that the quercetin-doped adhesive (500 µg/mL) preserved its bonding properties against collagenase ageing and inhibited the growth of S. mutans biofilm. Efficient bonding interface sealing ability, matrix metalloproteinase inhibition and acceptable biocompatibility were also achieved. Thus, a simple, safe and workable strategy was successfully developed to produce therapeutic adhesives for the extension of the service life of adhesive restorations.
不到 10 年，近一半的牙科修复体失败，大约 60%的临床时间用于更换这些牙科修复体。因此，应该对当代牙本质黏结剂进行改性，以治疗继发龋并防止黏结-牙本质界面的降解。为了实现这一目标，我们在本研究中通过将槲皮素（一种天然来源的植物提取物）掺入商业黏结剂中，开发了一种多功能治疗性黏结剂，浓度分别为 100、500 和 1000μg/mL。未改性的黏结剂作为对照。我们综合评估了载有槲皮素的黏结剂的抗变形链球菌生物膜的抗菌能力、转化率、微拉伸黏结强度、失效模式、原位酶谱、纳米渗漏表达和细胞毒性。结果表明，载有槲皮素的黏结剂（500μg/mL）保持了其胶原酶老化的黏结性能，并抑制了变形链球菌生物膜的生长。还实现了有效的黏结界面密封能力、基质金属蛋白酶抑制作用和可接受的生物相容性。因此，成功开发了一种简单、安全且可行的策略来制备治疗性黏结剂，以延长黏结修复体的使用寿命。
