Moreno-Grúa Elena, Pérez-Fuentes Sara, Muñoz-Silvestre Asunción, Viana David, Fernández-Ros Ana B, Sanz-Tejero Celia, Corpa Juan M, Selva Laura
Biomedical Research Institute (PASAPTA-Pathology Group), Facultad de Veterinaria, Universidad CEU Cardenal Herrera, CEU Universities, Valencia, Spain.
EXOPOL Autovacunas y Diagnóstico, Zaragoza, Spain.
Front Microbiol. 2018 Aug 14;9:1812. doi: 10.3389/fmicb.2018.01812. eCollection 2018.
Infections caused by methicillin-resistant (MRSA) have been a growing problem in human medicine since the 1960s, and more recently in veterinary medicine with the appearance of livestock-associated MRSA (LA-MRSA). Nevertheless, information about the presence of MRSA in rabbits is quite scarce since only one LA-MRSA identification has been previously reported. The present study aimed to determine genotypic characterization by verifying the presence of resistance determinants, virulence, and toxin genes of different strains that cause lesions in rabbits, and their phenotypic traits based on the antimicrobial susceptibility profile. The analysis of 240 isolates obtained from different lesion types collected from 89 Spanish and Portuguese rabbit commercial farms in the last 4 years (2014-2017) was performed. The methicillin-resistant gene A was found in 11.25% of the studied isolates (27 of 240) from 19 farms (13 Spanish and 6 Portuguese). Staphylococcal cassette chromosome (SCC) typing predominantly revealed type III ( = 15). Additionally, three MRSA isolates carrying the C gen were detected in samples from three different farms (two Spanish and one Portuguese). None of the 30 MRSA isolates was -positive or -positive. After the multilocus sequence typing (MLST) procedure, 16 belonged to ST2855, 6 to ST146, 6 to ST398, and 2 ST4774. No ST121 isolate was -positive. ST398 and ST4774 isolates lacked the immune-evasion-cluster (IEC) genes. ST2855 strains were associated with the presence only of the gene, and ST146 isolates were ascribed to IEC type E. Therefore, this is the first description of LA-MRSA from rabbits belonging to ST2855. Interestingly, one ST2855 and two ST4774 isolates were C-positive, which could act as a C-MRSA reservoir. More studies are needed to further characterize these isolates and their relationship with humans and other animal species.
自20世纪60年代以来，耐甲氧西林金黄色葡萄球菌（MRSA）引起的感染在人类医学中一直是一个日益严重的问题，最近在兽医学中随着与家畜相关的MRSA（LA-MRSA）的出现也成为问题。然而，关于兔体内MRSA存在情况的信息相当稀少，因为此前仅报道过一例LA-MRSA鉴定。本研究旨在通过验证引起兔病变的不同菌株的耐药决定因素、毒力和毒素基因的存在情况以及基于抗菌药物敏感性谱的表型特征，来确定其基因型特征。对过去4年（2014 - 2017年）从89个西班牙和葡萄牙兔商业养殖场收集的不同病变类型中获得的240株分离株进行了分析。在来自19个养殖场（13个西班牙养殖场和6个葡萄牙养殖场）的研究分离株（240株中的27株）中，发现11.25%含有耐甲氧西林基因A。葡萄球菌盒式染色体（SCC）分型主要显示为III型（= 15）。此外，在来自三个不同养殖场（两个西班牙养殖场和一个葡萄牙养殖场）的样本中检测到三株携带C基因的MRSA分离株。30株MRSA分离株中无一株为 -阳性或 -阳性。经过多位点序列分型（MLST）程序，16株属于ST2855，6株属于ST146，6株属于ST398，2株属于ST4774。没有ST121分离株为 -阳性。ST398和ST4774分离株缺乏免疫逃避簇（IEC）基因。ST28
