Teng Ke, Tan Penghui, Guo Weier, Yue Yuesen, Fan Xifeng, Wu Juying
Beijing Research and Development Center for Grass and Environment, Beijing Academy of Agriculture and Forestry Sciences, Beijing, China.
Turfgrass Research Institute, Beijing Forestry University, Beijing, China.
Front Plant Sci. 2018 Aug 14;9:1159. doi: 10.3389/fpls.2018.01159. eCollection 2018.
Growing evidence indicates that some grass species are more tolerant to various abiotic and biotic stresses than many crops. Zinc finger proteins play important roles in plant abiotic and biotic stresses. Although genes coding for these proteins have been cloned and identified in various plants, their function and underlying transcriptional mechanisms in the halophyte are barely known. In the present study, was isolated from using RACE method. Quantitative real time PCR results revealed that the expression of was much higher in leaf than in root and stem tissues, and induced by salt, cold or ABA treatment. The subcellular localization assay demonstrated that ZjZFN1 was localized to the nucleus. Expression of the in improved seed germination and enhanced plant adaption to salinity stress with improved percentage of green cotyledons and growth status under salinity stress. Physiological and transcriptional analyses suggested that ZjZFN1 might, at least in part, influence reactive oxygen species accumulation and regulate the transcription of salinity responsive genes. Furthermore, RNA-sequencing analysis of -overexpressing plants revealed that may serve as a transcriptional activator in the regulation of stress responsive pathways, including phenylalanine metabolism, α-linolenic acid metabolism and phenylpropanoid biosynthesis pathways. Taken together, these results provide evidence that is a potential key player in plants' tolerance to salt stress, and it could be a valuable gene in breeding projects.
越来越多的证据表明，一些草种比许多作物对各种非生物和生物胁迫具有更强的耐受性。锌指蛋白在植物非生物和生物胁迫中发挥着重要作用。尽管编码这些蛋白的基因已在多种植物中被克隆和鉴定，但其在盐生植物中的功能和潜在转录机制却鲜为人知。在本研究中，采用RACE方法从[植物名称]中分离得到[基因名称]。实时定量PCR结果显示，[基因名称]在叶片中的表达远高于根和茎组织，并受盐、冷或ABA处理诱导。亚细胞定位分析表明ZjZFN1定位于细胞核。在[植物名称]中过表达[基因名称]可提高种子萌发率，并增强植物对盐胁迫的适应性，盐胁迫下绿色子叶的比例和生长状态均有所改善。生理和转录分析表明，ZjZFN1可能至少部分影响活性氧的积累，并调节盐响应基因的转录。此外，对过表达[基因名称]的植物进行RNA测序分析发现，[基因名称]可能作为转录激活因子参与调控胁迫响应途径，包括苯丙氨酸代谢、α-亚麻酸代谢和苯丙烷生物合成途径。综上所述，这些结果证明[基因名称]是植物耐盐胁迫的潜在关键因子，在[植物名称]育种项目中可能是一个有价值的基因。
