Yilmaz Ayça Dilara, Yazicioglu Duygu, Tüzüner Öncül Ayşegül Mine, Yilmaz Erkan, Ereş Gülden
Molecular Biology Laboratory, Faculty of Dentistry, Ankara University, Emniyet Mah. İncitaş Sokak. Sabancı Kız Yurdu karşısı, Yenimahalle, Ankara, Turkey.
Private Practice, Oral and Maxillofacial Surgery, Ankara, Turkey.
Mol Biol Rep. 2018 Dec;45(6):1839-1848. doi: 10.1007/s11033-018-4330-5. Epub 2018 Aug 28.
Genetic variations might play a role in susceptibility to temporomandibular joint internal derangement (TMJ-ID) and osteoarthritis of the joint (TMJOA). Vitamin D receptor (VDR) polymorphisms have been shown to be associated with disc degeneration-linked pathologies, particularly osteoarthritis (OA). The aim of this study was to evaluate whether VDR polymorphisms present susceptibility to TMJ-ID/TMJOA. The study included 49 unrelated TMJ-ID patients with OA (31.7 ± 7.9) that were grouped and evaluated as having anterior disk displacement with reduction (ADDwR, n = 24) (31.58 ± 8.25) and without reduction (ADDwoR, n = 25) (31.8 ± 7.53) and 70 healthy controls (28.22 ± 5.9). DNA was extracted from blood samples using the standard proteinase K/phenol-chloroform method. Apa1 and Taq1 polymorphisms were investigated using a polymerase chain reaction-based restriction fragment length polymorphism assay. When TMJ-ID patients, ADDwR cases and ADDwoR cases versus healthy controls were compared, Apa1 Aa genotype compared to AA genotype had odds ratios of 1.65, 1.79 and 1.64 respectively (p > 0.05). In TMJ-ID women versus healthy women Aa genotype had 2.06 fold (p = 0.15) odds compared to AA genotype. Taq1 results showed that in TMJ-ID patients and ADDwoR cases the Tt genotype had odds ratios of 0.63 and 0.44 fold (p > 0.05) respectively. In TMJ-ID women the Tt and tt genotypes had odds ratios of 0.53 and 0.73 (p > 0.05). Combined VDR genotypes revealed that AATT had a 3.3 fold (p = 1.21) odds ratio while AATt had a 2.0 fold odds ratio (p = 0.29) (OR 0.59, 95% CI 0.23-1.49, p = 0.26) compared to AaTt. Although our results do not confirm susceptibility of VDR polymorphisms to TMJ-ID/TMJOA ,this relation needs to be further evaluated in a large cohort study.
基因变异可能在颞下颌关节内紊乱（TMJ-ID）和关节骨关节炎（TMJOA）的易感性中起作用。维生素D受体（VDR）多态性已被证明与椎间盘退变相关疾病，特别是骨关节炎（OA）有关。本研究的目的是评估VDR多态性是否会导致TMJ-ID/TMJOA易感性增加。该研究纳入了49例患有OA的非亲属TMJ-ID患者（31.7±7.9岁），这些患者被分组并评估为可复性盘前移位（ADDwR，n = 24）（31.58±8.25岁）和不可复性盘前移位（ADDwoR，n = 25）（31.8±7.53岁），以及70名健康对照者（28.22±5.9岁）。使用标准的蛋白酶K/酚-氯仿法从血样中提取DNA。使用基于聚合酶链反应的限制性片段长度多态性分析来研究Apa1和Taq1多态性。当比较TMJ-ID患者、ADDwR病例和ADDwoR病例与健康对照者时，与AA基因型相比，Apa1 Aa基因型的比值比分别为1.65、1.79和1.64（p>0.05）。在TMJ-ID女性与健康女性中，Aa基因型与AA基因型相比的比值比为2.06倍（p = 0.15）。Taq1结果显示，在TMJ-ID患者和ADDwoR病例中，Tt基因型的比值比分别为0.63倍和0.44倍（p>0.05）。在TMJ-ID女性中，Tt和tt基因型的比值比分别为0.53和0.73（p>0.05）。联合VDR基因型显示，与AaTt相比，AATT的比值比为3.3倍（p = 1.21），而AATt的比值比为2.0倍（p = 0.29）（OR 0.59，95%CI 0.23-1.49，p = 0.26）。虽然我们的结果未证实VDR多态性与TMJ-ID/TMJOA易感性相关，但这种关系需要在大型队列研究中进一步评估。
