Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Gaziosmanpasa University, Tokat, Turkey.
Department of Veterinary Genetics, Faculty of Veterinary, Ondokuz Mayıs University, Samsun, Turkey; Department of Medical Biology, Faculty of Medicine, Gaziosmanpasa University, Tokat, Turkey.
Br J Oral Maxillofac Surg. 2021 Jun;59(5):555-560. doi: 10.1016/j.bjoms.2020.08.101. Epub 2020 Aug 28.
Vitamin D (VD) levels and several variants in the vitamin D receptor (VDR) gene are associated with the occurrence of diseases of the bones and cartilage. The aim of this research was to study and compare the association of the BsmI variant in the VDR gene as well as VD levels in disc displacement with reduction (DDR) between patients and healthy controls. This was a case-control study, in which 104 patients of DDR and 102 healthy individuals were studied. The Diagnostic Criteria for Temporomandibular Disorders (DC/TMD) was used to diagnose temporomandibular diseases. The VDR BsmI variant was investigated, after extraction of genomic DNA, by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), and the VD level in serum was measured. The serum VD level was significantly different between the patient and the control group (mean (SD) 13.20 (11.02) ng/mL versus 18.44 (10.03) ng/mL, respectively) (p=0.008). Serum VD assessment revealed that serious vitamin D deficiency was more prevalent in the patients than the controls (50.96% versus 21.56%) (p=0.00001). Logistic regression analysis revealed that the bb genotype and b allele carriers of VDR BsmI variant were significantly associated with increased risk of DDR (p=0.022 and p=0.01, respectively). VDR BsmI BB genotype was higher in the control group than the patient group (p=0.045). Genotype distributions for BsmI variant in the controls and the patients were confirmed using the Hardy-Weinberg equilibrium equation. The BsmI variant of the VDR gene and VD deficiency play role in DDR aetiopathogenesis in a Turkish population. Vitamin D level and VDR BsmI variation may be effective in a possible genetic-based DC/TMD Axis III to be created in the future.
维生素 D（VD）水平和维生素 D 受体（VDR）基因中的几种变体与骨骼和软骨疾病的发生有关。本研究旨在研究和比较维生素 D 受体基因中的 BsmI 变体以及椎间盘可复性前移位（DDR）患者与健康对照组之间的 VD 水平。这是一项病例对照研究，共纳入 104 例 DDR 患者和 102 例健康对照者。采用颞下颌关节紊乱病诊断标准（DC/TMD）诊断颞下颌疾病。提取基因组 DNA 后，采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）检测 VDR BsmI 变体，并用酶联免疫吸附法检测血清 VD 水平。患者组和对照组之间血清 VD 水平存在显著差异（分别为 13.20（11.02）ng/mL 和 18.44（10.03）ng/mL）（p=0.008）。血清 VD 评估显示，患者中严重维生素 D 缺乏的比例明显高于对照组（50.96%对 21.56%）（p=0.00001）。Logistic 回归分析显示，VDR BsmI 变体的 bb 基因型和 b 等位基因携带者患 DDR 的风险显著增加（p=0.022 和 p=0.01）。VDR BsmI 基因型在对照组中的分布高于患者组（p=0.045）。对照组和患者组的 BsmI 变体基因型分布均符合 Hardy-Weinberg 平衡方程。维生素 D 受体基因 BsmI 变体和 VD 缺乏在土耳其人群中与 DDR 的发病机制有关。VD 水平和 VDR BsmI 变异可能在未来创建基于遗传的 DC/TMD 轴 III 中发挥作用。
