1Department of Entomology and Plant Pathology, University of Tennessee, Knoxville, TN 37996, USA.
†Present address: Life Science and Technology Center, China National Seed Group Company Limited, Wuhan 430075, PR China.
J Gen Virol. 2018 Oct;99(10):1418-1424. doi: 10.1099/jgv.0.001139. Epub 2018 Aug 29.
Analysis of transcriptome sequence data from eggs and second-stage juveniles (J2s) of sugar beet cyst nematode (SBCN, Heterodera schachtii) identified the full-length genome of a positive-sense single-stranded RNA virus, provisionally named sugar beet cyst nematode virus 1 (SBCNV1). The SBCNV1 sequence was detected in both eggs and J2s, indicating its possible vertical transmission. The 9503-nucleotide genome sequence contains a single long open reading frame, which was predicted to encode a polyprotein with conserved domains for picornaviral structural proteins proximal to its amino terminus and RNA helicase, cysteine proteinase and RNA-dependent RNA polymerase (RdRp) conserved domains proximal to its carboxyl terminus, hallmarks of viruses belonging to the order Picornavirales. Phylogenetic analysis of the predicted SBCNV1 RdRp amino acid sequence indicated that the SBCNV1 sequence is most closely related to members of the family Secoviridae, which includes genera of nematode-transmitted plant-infecting viruses. SBCNV1 represents the first fully sequenced viral genome from SBCN.
从甜菜胞囊线虫（SBCN， Heterodera schachtii）的卵和第二代幼虫（J2）的转录组序列数据中分析，鉴定出一种正链单链 RNA 病毒的全长基因组，暂命名为甜菜胞囊线虫病毒 1（SBCNV1）。SBCNV1 序列在卵和 J2 中均被检测到，表明其可能垂直传播。该 9503 核苷酸基因组序列包含一个单一的长开放阅读框，预测编码一种多蛋白，其氨基末端附近具有小 RNA 病毒结构蛋白的保守结构域，羧基末端附近具有 RNA 解旋酶、半胱氨酸蛋白酶和 RNA 依赖性 RNA 聚合酶（RdRp）的保守结构域，这些都是属于小 RNA 病毒目的病毒的特征。预测的 SBCNV1 RdRp 氨基酸序列的系统发育分析表明，SBCNV1 序列与线虫传播的植物感染病毒的科（家族）的成员最为密切相关。SBCNV1 代表了 SBCN 中第一个完全测序的病毒基因组。
