Reyes-Proaño Edison, Griffin Anna M, Duarte Aida, Sheng Hongyan, Schroeder Brenda K, Murray Timothy D, Karasev Alexander V
Department of Entomology, Plant Pathology and Nematology, University of Idaho, Moscow, ID 83844, USA.
Plant Pathogen Confirmatory Diagnostics Laboratory, USDA-APHIS-PPQ, Laurel, MD 20708, USA.
Viruses. 2025 Jul 23;17(8):1032. doi: 10.3390/v17081032.
A novel barna-like virus was found to be associated with field-collected plant-parasitic nematodes. The positive-sense, single-stranded RNA genome of this virus, named Afrina barna-like virus (AfBLV), comprises 4020 nucleotides encoding four open reading frames (ORFs). ORF 1 encodes a protein product spanning a transmembrane, a peptidase, and VPg domains, whereas an overlapping ORF 2 encodes an RNA-dependent RNA polymerase (RdRP). ORF2 may be expressed via a -1 translational frameshift. In phylogenetic reconstructions, the RdRP of AfBLV was placed inside a separate clade of barna and barna-like viruses related to but distinct from the genera in the and families, within the overall lineage of . ORF 3 of AfBLV encodes a protein product of 206 amino acids (aa) long with homology to a putative protein encoded by a similarly positioned gene of an uncharacterized virus sequence identified previously as Barnaviridae sp. ORF 4 encodes a 161 aa protein with no significant similarities to sequences in the GenBank databases. AfBLV is the first barnavirus found in a nematode. Sequence comparisons of the AfBLV genome and genomes of other barna-like viruses suggested that a recombination event was involved in the evolution of AfBLV. Analyses of the phylogeny of RdRPs and genome organizations of barna-like and solemo-like viruses support the re-classification of and genera as members of the family.
一种新型类巴纳病毒被发现与田间采集的植物寄生线虫有关。这种病毒名为非洲类巴纳病毒（AfBLV），其正义单链RNA基因组由4020个核苷酸组成，编码四个开放阅读框（ORF）。ORF1编码一个跨越跨膜、肽酶和VPg结构域的蛋白质产物，而重叠的ORF2编码一种RNA依赖性RNA聚合酶（RdRP）。ORF2可能通过 -1 移码翻译来表达。在系统发育重建中，AfBLV的RdRP位于巴纳病毒和类巴纳病毒的一个单独分支内，与 科和 科中的属相关但不同，属于整个 谱系。AfBLV的ORF3编码一个206个氨基酸（aa）长的蛋白质产物，与先前鉴定为巴纳病毒科属的未表征病毒序列中类似定位基因编码的假定蛋白质具有同源性。ORF4编码一个161 aa的蛋白质，与GenBank数据库中的序列没有显著相似性。AfBLV是在一种线虫中发现的第一种巴纳病毒。AfBLV基因组与其他类巴纳病毒基因组的序列比较表明，AfBLV的进化涉及一次重组事件。对类巴纳病毒和类索勒莫病毒的RdRPs系统发育和基因组组织的分析支持将 属和 属重新分类为 科的成员。
