多萜醇在磷脂膜中的定位。一项电子自旋共振自旋标记研究。

Localization of dolichols in phospholipid membranes. An ESR spin label study.

作者信息

Lai C S, Schutzbach J S

出版信息

FEBS Lett. 1986 Jul 28;203(2):153-6. doi: 10.1016/0014-5793(86)80733-5.

Abstract

We have used ESR methods employing spin-labeled stearates to investigate the effects of dolichol on the motion of lipid molecules in phospholipid membranes of phosphatidylethanolamine and phosphatidylcholine. The ESR spectra show that the presence of dolichol affects the motion of the spin probes at carbon-16, but not at carbon-5. Similar results are obtained with phospholipid membranes comprising only phosphatidylcholine. It is suggested that dolichol molecules are present mainly in the lipid core region of phospholipid membranes.

摘要

我们运用了采用自旋标记硬脂酸盐的电子自旋共振（ESR）方法，来研究多萜醇对磷脂酰乙醇胺和磷脂酰胆碱磷脂膜中脂质分子运动的影响。ESR光谱表明，多萜醇的存在会影响碳-16位自旋探针的运动，但不影响碳-5位自旋探针的运动。仅由磷脂酰胆碱组成的磷脂膜也得到了类似结果。这表明多萜醇分子主要存在于磷脂膜的脂质核心区域。