Wang Jun-Juan, Li Si-Han, Li Ai-Li, Zhang Qi-Min, Ni Wei-Wei, Li Mei-Na, Meng Xiang-Chen, Li Chun, Jiang Shi-Long, Pan Jian-Cun, Li Yuan-Yuan
a Key laboratory of Dairy Science, Ministry of Education, and Food Science College , Northeast Agriculture University , Harbin , China.
b Heilongjiang Feihe Dairy Co., Ltd , Harbin , China.
Biosci Biotechnol Biochem. 2018 Nov;82(11):1955-1963. doi: 10.1080/09168451.2018.1495551. Epub 2018 Aug 29.
This study aims to investigate the correlation between the ability of L. acidophilus to modulate miRNA expression and prevent Th17-dominated β-lactoglobulin (β-Lg) allergy. In vitro immunomodulation was evaluated by measuring splenocyte proliferation, Th17-related immune response and miRNA expression in β-Lg-sensitized splenocytes cultured with live L. acidophilus. Next, the allergic mouse model was used to evaluate anti-allergy capability of lactobacilli. The β-Lg challenge led to induction of up-regulation of miR-146a, miR-155, miR-21 and miR-9 expression in both in vivo and in vitro, along with increased Th17-related cytokine levels and mRNA expression of RORγt and IL-17. However, treatment of live L. acidophilus significantly suppressed hypersensitivity responses and Th17 cell differentiation. Moreover, administration of live L. acidophilus reduced expression of four miRNAs, especially miR-146a and miR-155. In addition, the decreased expression of the miRNAs in the spleen of the L. acidophilus-treated group was closely associated with decrease of IL-17 and RORγt mRNA expression.
本研究旨在探讨嗜酸乳杆菌调节miRNA表达的能力与预防以Th17为主导的β-乳球蛋白（β-Lg）过敏之间的相关性。通过测量用活嗜酸乳杆菌培养的β-Lg致敏脾细胞中的脾细胞增殖、Th17相关免疫反应和miRNA表达，对体外免疫调节进行评估。接下来，使用过敏性小鼠模型评估乳酸菌的抗过敏能力。β-Lg激发在体内和体外均导致miR-146a、miR-155、miR-21和miR-9表达上调，同时Th17相关细胞因子水平以及RORγt和IL-17的mRNA表达增加。然而，活嗜酸乳杆菌处理显著抑制了超敏反应和Th17细胞分化。此外，活嗜酸乳杆菌给药降低了四种miRNA的表达，尤其是miR-146a和miR-155。另外，嗜酸乳杆菌处理组脾脏中miRNA表达的降低与IL-17和RORγt mRNA表达的降低密切相关。
