Ni W-W, Zhang Q-M, Zhang X, Li Y, Yu S-S, Wu H-Y, Chen Z, Li A-L, Du P, Li C
Key Laboratory of Dairy Science, Food Science College, Northeast Agriculture University, Northeast Agriculture University, Harbin, China.
Key Laboratory of Dairy Science, Food Science College, Northeast Agriculture University, Northeast Agriculture University, Harbin, China.
Allergol Immunopathol (Madr). 2020 Mar-Apr;48(2):149-157. doi: 10.1016/j.aller.2019.06.002. Epub 2019 Aug 30.
β-lactoglobulin (β-Lg)-sensitized mice model was employed to investigate the correlation between Lactobacillus acidophilus KLDS 1.0738 (Lap KLDS 1.0738) modulating gut microbiota and inducting Toll-like receptors (TLRs) expression.
The alterations of mice fecal microbiota were analyzed by 16S rRNA gene sequencing. The serum cytokines production and TLR4/NF-κB mRNA expression in the colon tissues were measured by ELISA kit and quantitative RT-PCR, respectively.
The results showed that Lap KLDS 1.0738 pretreatment attenuated β-Lg-induced hypersensitivity, accompanied with a diminished expression of TLR4/NF-κB signaling. Moreover, oral administration of Lap KLDS 1.0738 improved the richness and diversity of fecal microbiota, which was characterized by fewer Proteobacteria phylum and Helicobacteraceae family, and higher Firmicutes phylum and Lachnospiraceae family than allergic group. Notably, TLR4/NF-κB expression was positively correlated with the family of Helicobacteraceae in allergic group, but negatively correlated with the family of Lachnospiraceae, Ruminococcaceae and anti-inflammatory cytokines level. A significant positive correlation was observed between TLR4/NF-κB expression and the production of histamine, total IgE and pro-inflammatory cytokines.
Intake of Lap KLDS 1.0738 can influence the gut bacterial composition, which might result in recognizing TLRs signaling so as to inhibit allergic response.
采用β-乳球蛋白（β-Lg）致敏小鼠模型，研究嗜酸乳杆菌KLDS 1.0738（Lap KLDS 1.0738）调节肠道微生物群与诱导Toll样受体（TLRs）表达之间的相关性。
通过16S rRNA基因测序分析小鼠粪便微生物群的变化。分别用ELISA试剂盒和定量RT-PCR检测结肠组织中血清细胞因子的产生和TLR4/NF-κB mRNA的表达。
结果表明，Lap KLDS 1.0738预处理可减轻β-Lg诱导的超敏反应，同时TLR4/NF-κB信号表达降低。此外，口服Lap KLDS 1.0738可改善粪便微生物群的丰富度和多样性，其特征是变形菌门和螺杆菌科较少，厚壁菌门和毛螺菌科比过敏组更高。值得注意的是，过敏组中TLR4/NF-κB表达与螺杆菌科呈正相关，但与毛螺菌科、瘤胃球菌科和抗炎细胞因子水平呈负相关。TLR4/NF-κB表达与组胺、总IgE和促炎细胞因子的产生之间存在显著正相关。
摄入Lap KLDS 1.0738可影响肠道细菌组成，这可能导致识别TLRs信号从而抑制过敏反应。
