Department of Oral and Maxillofacial Surgery.
Department of Tumor Pathology, Institute of Oncology.
J Craniofac Surg. 2018 Oct;29(7):1794-1798. doi: 10.1097/SCS.0000000000004934.
In this study, we compared the bone-healing effects of the local application of platelet-rich fibrin (PRF) and hyaluronic acid (HA) to bilateral tibial fractures in rats. Twenty-three adult male Sprague-Dawley rats were used. Twenty-two animals were randomly allocated to a control group (n = 6) and 2 study groups: PRF (n = 8) and HA (n = 8). The 23rd rat was used as a donor to obtain PRF. Each group was divided into 2 subgroups for histomorphometric and radiologic assessments at 2 and 6 weeks. Foreign body reaction, necrosis, inflammation, new bone formation, and fibrosis were investigated as bone healing parameters in terms of histopathologic analysis. The difference between the groups for these parameters was evaluated. The radiologic evaluation was performed by comparing the 3-dimensional reconstruction images of the fracture sites between the study and control groups. Histomorphometric evaluation showed that at 2 weeks postoperatively, the control group showed lesser bone formation (26.1 ± 6.6%) when compared to the study (HA: 54.7 ± 9.7%; PRF: 75.3 ± 19.2%) groups and PRF group showed highest total ossification. At 6 weeks postoperatively the PRF group showed lesser total ossification (50.7 ± 28.2%) when compared to control (76.3 ± 21.7%) and HA group. The HA (88.8 ± 13.3%) showed highest total ossification. In the control group, fibrosis was more prominent at week 6, whereas in the HA and PRF groups the amount of ossification increased. In contrast to histopathologic healing, radiologic bone healing did not differ significantly among the study and control groups 2 weeks after surgery, whereas at 6 weeks, the results of radiologic bone formation were in accordance with those of histopathologic bone healing.
在这项研究中，我们比较了局部应用富血小板纤维蛋白（PRF）和透明质酸（HA）对大鼠双侧胫骨骨折的骨愈合效果。使用了 23 只成年雄性 Sprague-Dawley 大鼠。22 只动物被随机分配到对照组（n = 6）和 2 个研究组：PRF（n = 8）和 HA（n = 8）。第 23 只大鼠被用作获取 PRF 的供体。每组分为 2 个亚组，在 2 周和 6 周时进行组织形态计量学和放射学评估。通过组织病理学分析，将异物反应、坏死、炎症、新骨形成和纤维化作为骨愈合参数进行研究。评估了这些参数在各组之间的差异。通过比较研究组和对照组骨折部位的三维重建图像，对放射学评估进行了评估。组织形态计量学评估显示，术后 2 周时，与研究组（HA：54.7 ± 9.7%；PRF：75.3 ± 19.2%）相比，对照组的骨形成较少（26.1 ± 6.6%），PRF 组的总骨化最高。术后 6 周时，PRF 组的总骨化（50.7 ± 28.2%）较对照组（76.3 ± 21.7%）和 HA 组低。HA（88.8 ± 13.3%）的总骨化最高。在对照组中，第 6 周时纤维化更明显，而在 HA 和 PRF 组中，骨化量增加。与组织病理学愈合相反，术后 2 周时，研究组和对照组的放射学骨愈合无显著差异，而术后 6 周时，放射学骨形成的结果与组织病理学骨愈合的结果一致。
