Department Orthopaedics and Traumatology, Çerkezköy State Hospital, Tekirdağ, Turkey.
Department Orthopaedics and Traumatology, Hacettepe University, Ankara, Turkey.
Acta Orthop Traumatol Turc. 2023 Sep;57(5):229-236. doi: 10.5152/j.aott.2023.22163.
This study aimed to investigate the role of an exogenous Epidermal Growth Factor and a hyaluronic acid-based scaffold on fracture healing in a rat femoral fracture model Methods: Forty-eight male Wistar-Albino rats, each weighing a mean 392 grams (range= 350-450 grams) and aged 8.2 months (6-9 months), were used for this experimental study. All surgical procedures were performed on the left femur by a single surgeon. An open femoral fracture was created in all rats. The animals were randomly divided into one of the four groups: Control (12), EGF (12), HA (12) and Combined (12). In the 4th and sixth weeks, samples were processed and analyzed using biomechanical and histological methods.
Fracture healing was significantly improved in the Combined group compared to the control one, EGF and HA groups in all parameters at both experimental time points. At the fourth and sixth weeks after surgery, fracture healing in the EGF and HA groups was significantly increased at histological evaluation compared to controls. In addition, compared with EGF, HA and Control groups, a significant difference in callus tissue was detected in the Combined group at 4 and 6-week time points in biomechanical features.
This study has shown that combining local EGF and HA scaffold accelerates bone healing and strengthens the bony callus histologically and biomechanically. Using EGF-HA combined scaffolds may represent a possible future strategy in trauma surgery.
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本研究旨在探讨外源性表皮生长因子和透明质酸支架在大鼠股骨骨折模型骨折愈合中的作用。
本实验研究使用了 48 只雄性 Wistar-Albino 大鼠，平均体重 392 克（范围 350-450 克），年龄 8.2 个月（6-9 个月）。所有手术均由一位外科医生在左侧股骨上进行。所有大鼠均造成开放性股骨骨折。动物随机分为四组之一：对照组（12 只）、EGF 组（12 只）、HA 组（12 只）和联合组（12 只）。在第 4 周和第 6 周，使用生物力学和组织学方法处理和分析样本。
与对照组相比，EGF 组和 HA 组在所有参数上均显著改善了骨折愈合。在手术后第 4 周和第 6 周，EGF 组和 HA 组的骨折愈合在组织学评估中明显优于对照组。此外，与 EGF、HA 和对照组相比，联合组在第 4 周和第 6 周的生物力学特征中，在骨痂组织方面存在显著差异。
本研究表明，局部应用 EGF 和 HA 支架可加速骨愈合，并在组织学和生物力学上增强骨性骨痂。使用 EGF-HA 联合支架可能代表创伤外科的一种未来策略。
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