Ismailov Z F, Smirnov S P, Tarasov V A
Genetika. 1986 May;22(5):777-86.
Four mutations were studied which lead to increasing the frequency of transposon Tn1 translocation into different replicons. These mutations (het1, het2, het3 and het4) increase the frequency of Tn1 translocation 10-20-fold. The het1 mutation is recessive and has been localized in the 90-94.5 min region of the bacterial chromosome. The mutation effects Tn1 transposition in the presence of F plasmid only. As we have demonstrated recently, F-plasmid inhibits Tn1 transposition in Escherichia coli cells. The het1 mutation eliminates this inhibition. Unlike het2, het3 and het4 mutations, het1 is responsible for resistance to male phages f1, f2, MS2 and inhibition of conjugative transfer in F+ bacteria.
对导致转座子Tn1易位到不同复制子频率增加的四种突变进行了研究。这些突变（het1、het2、het3和het4）使Tn1易位频率增加了10至20倍。het1突变是隐性的，已定位在细菌染色体的90 - 94.5分钟区域。该突变仅在F质粒存在时影响Tn1转座。正如我们最近所证明的，F质粒在大肠杆菌细胞中抑制Tn1转座。het1突变消除了这种抑制作用。与het2、het3和het4突变不同，het1负责对雄性噬菌体f1、f2、MS2的抗性以及对F + 细菌中接合转移的抑制。
