Elizbarashvili D A, Smirnov S P, Tarasov V A
Mol Gen Mikrobiol Virusol. 1985 Apr(4):35-9.
The efficiency of Tn1 transposition has been shown to increase considerably in course of bacterial conjugation. Usually, the frequency of Tn1 transposition from plasmid pSA2001, a derivative of RP4, into the chromosome never exceeded 0.1% per cell. Percentage of His+ transconjugants, marked by transposon Tn1 during conjugation between Hfr donor, carrying plasmid pSA2001, and auxotrophic recipient, was about 30%. Transposon Tn1 transfer into the recipient cells does not depend on the recA+ gene function in donor cells or on conjugative transfer of plasmid pSA2001. The transfer requires the recA+ gene function in recipients as well as the Hfr function in donor cells. Southern's blot-hybridization revealed the insertion of transposon Tn1 into the different sites of the chromosome of His+ transconjugants. The transposon inserted during conjugation retains the ability to potential further translocation into new sites on the chromosomal DNA.
已证明Tn1转座效率在细菌接合过程中会显著提高。通常，从质粒pSA2001（RP4的衍生物）到染色体的Tn1转座频率从未超过每个细胞0.1%。在携带质粒pSA2001的高频重组（Hfr）供体与营养缺陷型受体之间的接合过程中，由转座子Tn1标记的His⁺ 接合子的百分比约为30%。转座子Tn1转移到受体细胞中不依赖于供体细胞中的recA⁺ 基因功能或质粒pSA2001的接合转移。这种转移需要受体中的recA⁺ 基因功能以及供体细胞中的Hfr功能。Southern印迹杂交揭示了转座子Tn1插入到His⁺ 接合子染色体的不同位点。在接合过程中插入的转座子保留了进一步转移到染色体DNA新位点的潜在能力。
