Departamento de Genética, Escola Superior de Agricultura "Luiz de Queiroz", Universidade de São Paulo, 13418-900, Piracicaba, Brazil.
Institut National de la Recherche Agronomique (INRA), Centre National de Ressources Génomique Végétales, 31326, Castanet-Tolosan, France.
Sci Rep. 2018 Aug 29;8(1):13024. doi: 10.1038/s41598-018-31330-8.
Passiflora edulis is the most widely cultivated species of passionflowers, cropped mainly for industrialized juice production and fresh fruit consumption. Despite its commercial importance, little is known about the genome structure of P. edulis. To fill in this gap in our knowledge, a genomic library was built, and now completely sequenced over 100 large-inserts. Sequencing data were assembled from long sequence reads, and structural sequence annotation resulted in the prediction of about 1,900 genes, providing data for subsequent functional analysis. The richness of repetitive elements was also evaluated. Microsyntenic regions of P. edulis common to Populus trichocarpa and Manihot esculenta, two related Malpighiales species with available fully sequenced genomes were examined. Overall, gene order was well conserved, with some disruptions of collinearity identified as rearrangements, such as inversion and translocation events. The microsynteny level observed between the P. edulis sequences and the compared genomes is surprising, given the long divergence time that separates them from the common ancestor. P. edulis gene-rich segments are more compact than those of the other two species, even though its genome is much larger. This study provides a first accurate gene set for P. edulis, opening the way for new studies on the evolutionary issues in Malpighiales genomes.
西番莲是西番莲科中最广泛种植的物种，主要用于工业化果汁生产和新鲜水果消费。尽管它具有商业重要性，但人们对其基因组结构知之甚少。为了填补我们知识中的这一空白，构建了一个基因组文库，并对其进行了测序，现在已经完全测序了 100 多个大片段。测序数据是从长序列读取中组装的，结构序列注释预测了大约 1900 个基因，为后续的功能分析提供了数据。还评估了重复元件的丰富度。研究了西番莲与杨树和木薯共有的微同线性区域，杨树和木薯是两个具有完整测序基因组的相关锦葵科物种。总体而言，基因顺序得到了很好的保守，一些共线性的破坏被确定为重排，如倒位和易位事件。鉴于它们与共同祖先的分化时间很长，从比较基因组中观察到的西番莲序列之间的微同线性水平令人惊讶。与其他两个物种相比，西番莲基因丰富的片段更加紧凑，尽管其基因组要大得多。这项研究为西番莲提供了第一个准确的基因集，为锦葵科基因组的进化问题研究开辟了新的途径。
