Key Laboratory for Nano-Bio Interface Research, Division of Nanobiomedicine, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou, 215123, P.R. China.
Xi'an Jiaotong University, Xi'an, 710049, P.R. China.
Macromol Biosci. 2018 Nov;18(11):e1800086. doi: 10.1002/mabi.201800086. Epub 2018 Aug 29.
Basic fibroblast growth factor (bFGF) is an important protein for wound healing and angiogenesis in tissue engineering, but the lack of a viable delivery system hampers its clinical application. This study aims to maintain the long-term controlled release of bFGF by utilizing a collagen/heparin bi-affinity multilayer delivery system (CHBMDS), which is fabricated by the alternate deposition of negatively charged heparin, positively charged collagen, and CBD-bFGF (a collagen-binding domain [CBD] was fused into the native bFGF) via specific or electrostatic interaction. The results show that CHBMDS not only support localized and prolonged release of CBD-bFGF(over 35 days) but also lead to enhanced angiogenesis (higher density and larger diameter (≈70 µm) of newly formed blood vessels in subcutaneous tissue of SD rat after 5 weeks). This system could act as a versatile approach for bFGF delivery and further improve therapeutic efficacy for injured tissues.
碱性成纤维细胞生长因子（bFGF）是组织工程中伤口愈合和血管生成的重要蛋白，但缺乏可行的递送系统限制了其临床应用。本研究旨在通过利用胶原/肝素双亲和多层递药系统（CHBMDS）来维持 bFGF 的长期控释，该系统是通过带负电荷的肝素、带正电荷的胶原蛋白和 CBD-bFGF（将胶原结合结构域 [CBD] 融合到天然 bFGF 中）之间的特异性或静电相互作用，通过交替沉积制备而成。结果表明，CHBMDS 不仅支持 CBD-bFGF 的局部和长时间释放（超过 35 天），而且还导致血管生成增强（在 5 周后 SD 大鼠皮下组织中，新形成的血管密度更高，直径更大（≈70 µm））。该系统可作为 bFGF 递送的一种多功能方法，进一步提高受损组织的治疗效果。
