Erfanian Saeideh, Mostafaei Farhad, Ajalloueian Fatemeh, Baharvand Hossein, Rajabi Sarah, Ashtiani Mohammad Kazemi
Department of Tissue Engineering, Faculty of Basic Sciences and Advanced Technologies in Medicine, Royan Institute, ACECR, Tehran, Iran.
Animal Core Facility, Reproductive Biomedicine Research Center, Royan Institute for Animal Biotechnology, ACECR, Tehran, Iran.
Sci Rep. 2025 Apr 13;15(1):12719. doi: 10.1038/s41598-025-92319-8.
The efficacy of platelet-rich plasma (PRP) in enhancing muscle regeneration was limited due to uncontrolled delivery. This study focuses on modification of decellularized scaffolds derived from skeletal muscle extracellular matrix (ECM) by heparin, loading with PRP, to enhance endogenous cell recruitment in muscle regeneration. Here, we showed an optimal decellularization procedure that effectively removed cells while preserving the ECM components in skeletal muscle tissue and the macroporous scaffolds showed suitable physical and mechanical properties for muscle tissue engineering. Heparinized scaffold released platelet-derived growth factor (PDGF), fibroblast growth factor (FGF) and vascular endothelial growth factor (VEGF) in a sustained manner. Moreover, the scaffold increased the metabolic activity and myogenic differentiation of C2C12 cells. In addition, histological analysis revealed higher angiogenesis and more extensive migration of the host muscle cells in the volumetric muscle loss (VML) model. The heparinized scaffold facilitated the attraction and sustained release of growth factors from PRP, which in turn promoted the recruitment of the endothelial and muscle cells in the injured tissue that may have the potential to enhance the accessibility of decellularized scaffolds for off-the-shelf use and to treat muscle defects in clinical practice.
由于递送不受控制，富血小板血浆（PRP）在促进肌肉再生方面的疗效有限。本研究聚焦于用肝素修饰源自骨骼肌细胞外基质（ECM）的脱细胞支架，并负载PRP，以增强肌肉再生过程中的内源性细胞募集。在此，我们展示了一种优化的脱细胞程序，该程序能有效去除细胞，同时保留骨骼肌组织中的ECM成分，且大孔支架展现出适合肌肉组织工程的物理和力学性能。肝素化支架能持续释放血小板衍生生长因子（PDGF）、成纤维细胞生长因子（FGF）和血管内皮生长因子（VEGF）。此外，该支架增加了C2C12细胞的代谢活性和肌源性分化。另外，组织学分析显示，在容积性肌肉损失（VML）模型中，血管生成增加，宿主肌肉细胞迁移更广泛。肝素化支架促进了PRP中生长因子的吸引和持续释放，这反过来又促进了受损组织中内皮细胞和肌肉细胞的募集，这可能有潜力提高脱细胞支架的现货可用性，并在临床实践中治疗肌肉缺损。
