细胞的 SEM-Raman 图像细胞计量术。

SEM-Raman image cytometry of cells.

机构信息

Medical Cell Biophysics group, University of Twente, PO Box 217, 7500 AE Enschede, The Netherlands.

出版信息

Analyst. 2018 Sep 10;143(18):4495-4502. doi: 10.1039/c8an00955d.

Abstract

Correlative and integrated scanning electron microscopy (SEM) and Raman micro-spectroscopy is presented that enables the characterization and identification of different cancer and non-cancer cells through SEM-Raman image cytometry. The hybrid microscopy system enables the acquisition of high resolution SEM images of uncoated cells and the spatial correlation with chemical information as obtained from Raman micro-spectroscopic imaging. A sample preparation protocol and a workflow are presented that are compatible with the demands of hybrid SEM-Raman microscopy. Stainless steel cell substrates were used that are both conductive and give a low optical response in Raman scattering. Correlative and integrated SEM-Raman micro-spectroscopy is illustrated with cells from blood and cells from a SKBR-3 breast cancer cell line.

摘要

本文提出了一种相关和集成的扫描电子显微镜（SEM）和拉曼微光谱技术，通过 SEM-Raman 图像细胞计量学，可以对不同的癌症和非癌细胞进行特征描述和识别。该混合显微镜系统能够获取未镀膜细胞的高分辨率 SEM 图像，并与从拉曼微光谱成像中获得的化学信息进行空间相关。本文提出了一种与混合 SEM-Raman 显微镜需求兼容的样品制备方案和工作流程。使用了不锈钢细胞基底，它们既具有导电性，又在拉曼散射中具有低的光学响应。本文还结合血液细胞和 SKBR-3 乳腺癌细胞系的细胞，说明了相关和集成的 SEM-Raman 微光谱技术。