Department of Obstetrics and Gynecology, College of Medicine, National Cheng Kung University, Tainan, Taiwan.
Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, Tainan, Taiwan.
Cytoskeleton (Hoboken). 2019 Jan;76(1):137-142. doi: 10.1002/cm.21491. Epub 2018 Dec 22.
The sperm annulus, a septin-based ring structure, is important for reproductive physiology. It is composed of SEPT12-based septin core complex followed by assembling as octameric filament. In clinical examinations, mutations of Septin12 result in male infertility, immotile sperm, as well as sperm with defective annuli. The dynamic assembly of septin filaments is regulated by several post-translational modifications, including sumoylation, acetylation, and phosphorylation. Here, we briefly review the biological significance and the regulation of SEPT12 phosphorylation in the mammalian sperm physiology. During mammalian spermiogenesis, the phosphorylation of SEPT12 on Ser198 residue is important in regulating mammalian annulus architectures. SEPT12 phosphomimetic knock-in mice displayed poor male fertility due to weak sperm motility and loss of the sperm annulus. SEPT12 is phosphorylated via Protein kinase A (PKA), and its phosphorylation interfered with SEPT12 polymerization into complexes and filaments. Taken together, the phosphorylation status of SEPT12 is crucial for its function in regulating the mammalian sperm physiology.
精子环，一种基于 septin 的环状结构，对于生殖生理学很重要。它由基于 SEPT12 的 septin 核心复合物组成，然后组装成八聚体纤维。在临床检查中，Septin12 的突变导致男性不育、不动精子以及具有缺陷环的精子。septin 纤维的动态组装受到几种翻译后修饰的调节，包括 sumoylation、乙酰化和磷酸化。在这里，我们简要回顾了 SEPT12 磷酸化在哺乳动物精子生理中的生物学意义和调节作用。在哺乳动物精子发生过程中，Ser198 残基上 SEPT12 的磷酸化对于调节哺乳动物环的结构很重要。SEPT12 磷酸模拟敲入小鼠由于精子运动能力弱和精子环丢失而表现出较差的雄性生育能力。SEPT12 通过蛋白激酶 A（PKA）磷酸化，其磷酸化干扰 SEPT12 聚合为复合物和纤维。总之，SEPT12 的磷酸化状态对于其调节哺乳动物精子生理的功能至关重要。
