Canning P C, Roth J A, Deyoe B L
J Infect Dis. 1986 Sep;154(3):464-70. doi: 10.1093/infdis/154.3.464.
Brucella abortus releases two components (fractions 3b and 10) that inhibit the myeloperoxidase-H2O2-halide antibacterial system of bovine neutrophils by inhibiting degranulation. Fractions 3b and 10 were analyzed by high-performance liquid chromatography and thin-layer chromotography and compared with nucleotide and base standards. These investigations indicated that fraction 3b coeluted and comigrated with 5'-guanosine monophosphate (GMP), whereas fraction 10 coeluted and comigrated with adenine. We determined the biologic effects of GMP, adenine, and B. abortus fractions 3b and 10 on neutrophil functions. Iodination activity of neutrophils was inhibited to approximately 65% of control by GMP (0.04 mg/ml) and by fraction 3b (0.04 mg/ml). Iodination was also suppressed to approximately 80% of control by adenine (0.04 mg/ml) and by fraction 10 (0.04 mg/ml). These results suggest that B. abortus produces GMP and adenine and that these substances contribute to the intracellular survival of the bacteria.
流产布鲁氏菌释放出两种成分（组分3b和10），它们通过抑制脱颗粒作用来抑制牛中性粒细胞的髓过氧化物酶-H2O2-卤化物抗菌系统。通过高效液相色谱法和薄层色谱法对组分3b和10进行了分析，并与核苷酸和碱基标准品进行了比较。这些研究表明，组分3b与5'-鸟苷单磷酸（GMP）共洗脱和共迁移，而组分10与腺嘌呤共洗脱和共迁移。我们测定了GMP、腺嘌呤以及流产布鲁氏菌组分3b和10对中性粒细胞功能的生物学效应。GMP（0.04mg/ml）和组分3b（0.04mg/ml）将中性粒细胞的碘化活性抑制至对照的约65%。腺嘌呤（0.04mg/ml）和组分10（0.04mg/ml）也将碘化作用抑制至对照的约80%。这些结果表明，流产布鲁氏菌产生GMP和腺嘌呤，并且这些物质有助于细菌在细胞内存活。
