Compito G A, Wagner B J, Stach R W
J Neurochem. 1986 Aug;47(2):554-62. doi: 10.1111/j.1471-4159.1986.tb04535.x.
Recent studies with sympathetic neurons using radiolabeled nerve growth factor have indicated that a high-molecular-weight covalent complex is formed. This complex is between the nerve growth factor and the high-affinity (type I) receptor and occurs through the formation of a disulfide bond. Studies presented in the present article demonstrate a similar complex is formed on chicken embryonic sensory neurons. The formation of this complex is inhibited by the addition of unlabeled nerve growth factor, metabolic energy inhibitors (dinitrophenol and NaF), and of sulfhydryl reagents. On the other hand, formation of this complex is not inhibited by temperature, or by the addition of insulin or epidermal growth factor. The receptor involved in the covalent complex formation is the high-affinity (type I) receptor. The molecular weight of this complex is approximately 232,000 daltons. Evidence indicates that this covalent complex may be required for the biological activity of the nerve growth factor.
最近利用放射性标记的神经生长因子对交感神经元进行的研究表明，会形成一种高分子量的共价复合物。这种复合物存在于神经生长因子与高亲和力（I型）受体之间，是通过二硫键的形成而产生的。本文所展示的研究表明，在鸡胚感觉神经元上也会形成类似的复合物。添加未标记的神经生长因子、代谢能量抑制剂（二硝基苯酚和氟化钠）以及巯基试剂会抑制这种复合物的形成。另一方面，温度、胰岛素或表皮生长因子的添加不会抑制这种复合物的形成。参与共价复合物形成的受体是高亲和力（I型）受体。这种复合物的分子量约为232,000道尔顿。有证据表明，这种共价复合物可能是神经生长因子生物活性所必需的。
