Stach R W, Wagner B J, Ennulat D J, Perez-Polo J R
Int J Neurosci. 1985 Apr;26(1-2):87-94. doi: 10.3109/00207458508985608.
Nerve growth factor is a polypeptide hormone that is required for the normal growth and development of the embryonic sensory and sympathetic nervous systems. On these cells, there are two different receptors for the nerve growth factor. Recently, these receptors have been isolated from three cell types and shown to have essentially the same binding characteristics. Molecular weights for receptors from two of these cell types, embryonic sensory and rat pheochromocytoma cells, have been determined. In addition, the formation of a covalent nerve growth factor, nerve growth factor receptor complex, has been investigated on embryonic sensory and sympathetic neurons. The formation of this covalent complex containing 125I-beta nerve growth factor is prevented by the addition of excess unlabeled nerve growth factor or by the addition of sodium fluoride and dinitrophenol. This complex forms at 4 degrees, 22 degrees, and 37 degrees, indicating that it is occurring on the cell surface. A disulfide bond is involved in the formation of this covalent complex.
神经生长因子是一种多肽激素，对于胚胎感觉和交感神经系统的正常生长和发育是必需的。在这些细胞上，存在两种不同的神经生长因子受体。最近，这些受体已从三种细胞类型中分离出来，并显示出基本相同的结合特性。已确定其中两种细胞类型（胚胎感觉细胞和大鼠嗜铬细胞瘤细胞）的受体的分子量。此外，还对胚胎感觉神经元和交感神经元上共价神经生长因子 - 神经生长因子受体复合物的形成进行了研究。通过添加过量未标记的神经生长因子或添加氟化钠和二硝基苯酚，可以阻止含有125I-β神经生长因子的这种共价复合物的形成。这种复合物在4℃、22℃和37℃下形成，表明它发生在细胞表面。一个二硫键参与了这种共价复合物的形成。
