Herring F G, Lam E W, Burt H M
J Rheumatol. 1986 Jun;13(3):623-30.
The nature of the membranolytic interaction between monosodium urate monohydrate (MSUM) crystals and phospholipid membranes was studied using electron spin resonance. Two spin probe molecules were incorporated into intact human erythrocytes and incubated with MSUM crystals. The apparent increased fluidity of 5-doxyl stearic acid incorporated erythrocytes after a 2 h incubation with MSUM was probably due to an electrostatically induced redistribution of probe from the outer more rigid layer to the fluid inner leaflet via a flip-flop mechanism. It was suggested that the MSUM induced redistribution of cationic amphiphilic probe population in the whole erythrocyte was also due to an electrostatic interaction between negatively charged MSUM crystals and positively charged probe. Possible mechanisms of MSUM induced membranolysis are discussed.
利用电子自旋共振研究了一水合尿酸钠（MSUM）晶体与磷脂膜之间的膜溶解相互作用的性质。将两种自旋探针分子掺入完整的人红细胞中，并与MSUM晶体一起孵育。与MSUM孵育2小时后，掺入5-硬脂酰氧基硬脂酸的红细胞的表观流动性增加，这可能是由于探针通过翻转机制从较刚性的外层静电诱导重新分布到流动性较强的内层小叶。有人认为，MSUM诱导的阳离子两亲性探针群体在整个红细胞中的重新分布也是由于带负电荷的MSUM晶体与带正电荷的探针之间的静电相互作用。讨论了MSUM诱导膜溶解的可能机制。
