Ting-Beall H P, Burgess F M, Robertson J D
J Microsc. 1986 Jun;142(Pt 3):311-6. doi: 10.1111/j.1365-2818.1986.tb04286.x.
We have obtained high resolution electron microscopic images of complementary membrane surface replicas of purified microsomal vesicles from pig kidney outer medulla containing Na+,K+-ATPase. Ultra-rapid freezing of a membrane suspension was followed by fracturing and replicating of the liquid helium cooled specimen under ultra-high vacuum conditions free of hydrocarbon contaminants. The protoplasmic fracture faces are populated with intramembrane particles while the external fracture faces reveal complementary pits. This is the first demonstration of extended precise matching of individual intramembrane particles and their corresponding pits in biological membranes containing transmembrane proteins. The data are also consistent with the theory that the majority of Na+,K+-ATPase mass is located at the protoplasmic half of the membrane.
我们获得了来自猪肾外髓质含有Na +，K + -ATP酶的纯化微粒体囊泡互补膜表面复制品的高分辨率电子显微镜图像。对膜悬浮液进行超快速冷冻，然后在无碳氢化合物污染物的超高真空条件下对液氦冷却的样品进行断裂和复制。原生质断裂面上布满了膜内颗粒，而外部断裂面则显示出互补的凹坑。这是首次证明在含有跨膜蛋白的生物膜中，单个膜内颗粒与其相应凹坑之间存在精确的扩展匹配。这些数据也与大多数Na +，K + -ATP酶质量位于膜原生质一半的理论相一致。
