Benko D M, Gibson W
J Virol. 1986 Sep;59(3):703-13. doi: 10.1128/JVI.59.3.703-713.1986.
The lectin-binding properties and glycosidase sensitivities of the virion glycoproteins of primate cytomegaloviruses (CMVs) were examined. Three simian CMV (SCMV) strains, including Colburn, and four human CMV (HCMV) strains were compared. Their proteins were separated in denaturing polyacrylamide gels and electrotransferred onto nitrocellulose, and the glycosylated species were visualized with iodinated concanavalin A or wheat germ agglutinin (WGA). Virions of both HCMV and SCMV strains contained six principal and several minor lectin-reactive bands. Neuraminidase treatment abolished WGA binding and reduced the charge and charge heterogeneity of the SCMV (i.e., Colburn) virion glycoproteins and had a similar, although less dramatic, effect on those of HCMV. The specificities of concanavalin A and WGA in these assays were evaluated with endo-beta-N-acetylglucosaminidase H and endo-beta-N-acetylglucosaminidase F, and a combination of lectins and glycosidases was used to demonstrate that many of the primate CMV glycoproteins contain both high-mannose and complex, N-linked oligosaccharides. Results suggest that the HCMV virion glycoproteins are more extensively glycosylated or have more completely processed carbohydrate side chains, or both, than their SCMV counterparts.
对灵长类巨细胞病毒（CMV）病毒粒子糖蛋白的凝集素结合特性和糖苷酶敏感性进行了检测。比较了包括科尔本株在内的三株猿猴CMV（SCMV）毒株和四株人CMV（HCMV）毒株。将它们的蛋白质在变性聚丙烯酰胺凝胶中分离并电转移到硝酸纤维素膜上，用碘化刀豆球蛋白A或麦胚凝集素（WGA）对糖基化种类进行可视化。HCMV和SCMV毒株的病毒粒子均含有六条主要的和几条次要的凝集素反应带。神经氨酸酶处理消除了WGA结合，并降低了SCMV（即科尔本株）病毒粒子糖蛋白的电荷和电荷异质性，对HCMV病毒粒子糖蛋白也有类似但不太显著的影响。用内切β-N-乙酰葡糖胺糖苷酶H和内切β-N-乙酰葡糖胺糖苷酶F评估了这些测定中刀豆球蛋白A和WGA的特异性，并使用凝集素和糖苷酶的组合来证明许多灵长类CMV糖蛋白同时含有高甘露糖型和复杂的N-连接寡糖。结果表明，与SCMV对应物相比，HCMV病毒粒子糖蛋白的糖基化程度更高，或者碳水化合物侧链的加工更完全，或者两者兼而有之。
