Melnikov A A, Fodor I
Mol Gen Genet. 1986 May;203(2):312-5. doi: 10.1007/BF00333972.
A genetic procedure for selection of specific lambda clones, by homologous recombination between lambda clones from a gene clonotheque and sequences cloned into a plasmid, was developed. Resulting clones are isolated in transduction experiments by plating infected Escherichia coli cells under conditions selecting for the antibiotic resistance marker carried by the plasmid. The feasibility of the method was demonstrated in a model test system as well as by isolation of alpha-interferon-specific sequences from the human gene clonotheque.
开发了一种通过基因克隆文库中的λ克隆与克隆到质粒中的序列之间的同源重组来选择特定λ克隆的遗传方法。在转导实验中，通过在选择质粒携带的抗生素抗性标记的条件下铺板感染的大肠杆菌细胞来分离得到的克隆。该方法的可行性在模型测试系统中以及通过从人类基因克隆文库中分离α-干扰素特异性序列得到了证明。
