Greene R C, Krueger J H, Johnson J R
Mol Gen Genet. 1982;187(3):401-4. doi: 10.1007/BF00332618.
The position of the metJBLF gene cluster in the transducing phage lambda met102 was determined by ligation of its leftmost EcoRI fragment (102-1) to the lambda BCDEF (nin5) EcoRI fragment of lambda gtl (lambda BC) and characterization of the resultant recombinant phage. The new transducing phage carries about 6kb of bacterial DNA which contains the entire met gene cluster including the promoter of its rightmost member metF. Reasonable estimates of the coding capacity required for the four genes indicate that most of the bacterial DNA of the recombinant phage is occupied by the met gene cluster.
通过将转导噬菌体λmet102中最左侧的EcoRI片段（102 - 1）与λgtl的λBCDEF（nin5）EcoRI片段（λBC）连接，并对所得重组噬菌体进行鉴定，确定了metJBLF基因簇在转导噬菌体λmet102中的位置。新的转导噬菌体携带约6kb的细菌DNA，其中包含整个met基因簇，包括其最右侧成员metF的启动子。对这四个基因所需编码能力的合理估计表明，重组噬菌体的大部分细菌DNA被met基因簇占据。
