Wang Qing, Wang Shaowen, Zhang Yong, Yu Yepin, Zhao Huihong, Yang Huirong, Zheng Leyun, Yang Min, Qin Qiwei
College of Marine Sciences, South China Agricultural University, Guangzhou, 510642, People's Republic of China.
State Key Laboratory of Biocontrol, Institute of Aquatic Economic Animals and Guangdong Provincial Key Laboratory for Aquatic Economic Animals, School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, People's Republic of China.
Dev Comp Immunol. 2019 Jan;90:10-20. doi: 10.1016/j.dci.2018.08.015. Epub 2018 Aug 27.
Chemokines comprise a group of small molecular weight (6-14 kDa) cytokines; chemokine receptors are a superfamily of seven transmembrane domain G-coupled receptors. Both chemokines and their receptors have important roles in immune surveillance, inflammation, and development. Recently, 9 CXC chemokine ligands (CXCLs) and 8 CXC chemokine receptors (CXCRs) were identified and cloned from orange-spotted grouper (Epinephelus coioides) and annotated by phylogenetic and syntenic analyses. We detected mRNA transcripts for CXCLs and CXCRs in healthy tissues of E. coioides. Our data show that CXCL genes are highly expressed in the spleen, kidney and liver and that CXCR genes are ubiquitously expressed, rather than being expressed only in immune organs. Analysis of gene expression after Singapore grouper iridovirus infection indicated that CXCL and CXCR genes are regulated in a gene-specific manner. CXCL8 and CXCL12a were significantly upregulated in the spleen, kidney and liver of resistant fish, indicating potential roles in immunity against the pathogen. Additionally, CXCR4a was upregulated in all three organs in resistant fish, suggesting that CXCL8 or CXCL12a may participate in the immune response via interaction with CXCR4a. In addition, the new orange-spotted grouper receptor CXCR1b was found to be upregulated in the spleen and kidney of resistant fish, indicating that this receptor plays an important role in immune responses to viral infection. These results are valuable for comparative immunological studies and provide insight into the roles of these genes in viral infection.
趋化因子是一组小分子量（6 - 14 kDa）的细胞因子；趋化因子受体是一个具有七个跨膜结构域的G蛋白偶联受体超家族。趋化因子及其受体在免疫监视、炎症和发育中都发挥着重要作用。最近，从点带石斑鱼（Epinephelus coioides）中鉴定并克隆了9种CXC趋化因子配体（CXCLs）和8种CXC趋化因子受体（CXCRs），并通过系统发育和共线性分析进行了注释。我们在点带石斑鱼的健康组织中检测到了CXCLs和CXCRs的mRNA转录本。我们的数据表明，CXCL基因在脾脏、肾脏和肝脏中高度表达，而CXCR基因在全身广泛表达，并非仅在免疫器官中表达。对新加坡石斑鱼虹彩病毒感染后的基因表达分析表明，CXCL和CXCR基因以基因特异性方式受到调控。CXCL8和CXCL12a在抗性鱼的脾脏、肾脏和肝脏中显著上调，表明它们在抵抗病原体免疫中具有潜在作用。此外，抗性鱼的所有三个器官中CXCR4a均上调，表明CXCL8或CXCL12a可能通过与CXCR4a相互作用参与免疫反应。此外，发现新的点带石斑鱼受体CXCR1b在抗性鱼的脾脏和肾脏中上调，表明该受体在病毒感染的免疫反应中起重要作用。这些结果对于比较免疫学研究具有重要价值，并为这些基因在病毒感染中的作用提供了深入了解。
