六倍体吉富罗非鱼中复制的 Cxcr4/Cxcl12 基因的动态和差异表达有助于抗病毒反应。
Dynamic and Differential Expression of Duplicated Cxcr4/Cxcl12 Genes Facilitates Antiviral Response in Hexaploid Gibel Carp.
机构信息
State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, The Innovation Academy of Seed Design, Chinese Academy of Sciences, Graduate University of the Chinese Academy of Sciences, Wuhan, China.
Institute of Marine Biology, College of Oceanography, Hohai University, Nanjing, China.
出版信息
Front Immunol. 2020 Sep 11;11:2176. doi: 10.3389/fimmu.2020.02176. eCollection 2020.
Chemokine receptor and its ligand have evolved two paralogs in the teleost lineage. In this study, we have identified four duplicated and genes from hexaploid gibel carp, , respectively. s and s were dynamically and differentially expressed in immune-related tissues, and significantly up-regulated in head kidney and spleen after crucian carp herpesvirus (HV) infection. Blocking Cxcr4/Cxcl12 axis by injecting AMD3100 brought more severe bleeding symptom and lower survival rate in HV-infected fish. AMD3100 treatment also suppressed the up-regulation of key antiviral genes in head kidney and spleen, and resulted in more acute replication of HV in . Consistently, the similar suppression of up-regulated expression of key antiviral genes were also observed in CAB cells treated by AMD3100 after poly(I:C) stimulation. Finally, MAPK3 and JAK/STAT were identified as the possible pathways that Cxcr4s and Cxcl12s participate in to promote the antiviral response in .
趋化因子受体及其配体在硬骨鱼谱系中进化出了两个平行基因。本研究从三倍体银鲫中分别鉴定出了 4 个重复的 和 基因。s 和 s 在免疫相关组织中呈现动态和差异表达，在鲫鱼疱疹病毒（HV）感染后头肾和脾脏中显著上调。通过注射 AMD3100 阻断 Cxcr4/Cxcl12 轴，会导致 HV 感染鱼出现更严重的出血症状和更低的存活率。AMD3100 处理还抑制了头肾和脾脏中关键抗病毒基因的上调，导致 HV 在 中的复制更急性。同样，在 poly(I:C)刺激后用 AMD3100 处理 CAB 细胞也观察到关键抗病毒基因上调表达的类似抑制。最后，确定 MAPK3 和 JAK/STAT 是 Cxcr4s 和 Cxcl12s 参与促进 的抗病毒反应的可能途径。
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