Faculty of Biology, University of Freiburg, 79104 Freiburg, Germany; SGBM - Spemann Graduate School of Biology and Medicine, University of Freiburg, 79104 Freiburg, Germany; BIOSS - Centre for Biological Signalling Studies, University of Freiburg, 79104 Freiburg, Germany.
Faculty of Biology, University of Freiburg, 79104 Freiburg, Germany.
Acta Biomater. 2018 Oct 1;79:276-282. doi: 10.1016/j.actbio.2018.08.032. Epub 2018 Aug 28.
Molecular traps can control activity and abundance of many biological factors. Here, we report the development of a generic opto-trap to reversibly bind and release biomolecules with high spatiotemporal control by illumination with non-invasive and cell-compatible red and far-red light. We use the Arapidopsis thaliana photoreceptor phytochrome B to regulate the release of diverse proteins from a variety of material scaffolds. Fusion of a short 100 amino acids "PIF-tag", derived from the phytochrome interacting factor 6, renders arbitrary molecules opto-trap-compatible. Reversible opto-trapping of target molecules enables novel possibilities for future developments in diagnostics, therapeutics, and basic research.
The investigation of cellular signaling events or the development of complex therapeutics and integrative diagnostic devices requires the deliberate control of biomolecule abundance and activity. During recent years, the use of natural photoreceptors within cells leveraged the control of diverse cellular events, benefiting from the superior spatial and temporal control characteristics of light as compared to conventional chemical stimuli. Concurrently, biological switches entailing intrinsic compatibility toward biological environments increasingly found application in biohybrid materials. We employ the plant red/far-red photoreceptor phytochrome B, which reversibly interacts with its phytochrome interacting factors (PIFs), for developing a generic opto-trap. This platform allows the use of red and far-red light to spatiotemporally control binding and release of arbitrary PIF-fused biomolecules from various material scaffolds.
分子陷阱可以控制许多生物因素的活性和丰度。在这里，我们报告了一种通用的光阱的开发，该光阱可以通过使用非侵入性和细胞兼容的红光和远红光进行照射，实现高时空控制，可逆地结合和释放生物分子。我们使用拟南芥光受体光敏色素 B 来调节各种蛋白质从各种材料支架中的释放。融合一个短的 100 个氨基酸的“PIF 标签”，来源于光相互作用因子 6，使任意分子都与光陷阱兼容。靶分子的可逆光捕获为未来在诊断、治疗和基础研究中的发展提供了新的可能性。
细胞信号事件的研究或复杂治疗和综合诊断设备的开发需要故意控制生物分子的丰度和活性。近年来，细胞内天然光受体的使用利用光的优越时空控制特性，控制了多种细胞事件，从而受益于光相比传统化学刺激物。同时，内在兼容生物环境的生物开关越来越多地应用于生物混合材料中。我们使用植物的红/远红光光受体光敏色素 B，它与它的光敏色素相互作用因子（PIFs）可逆相互作用，开发了一种通用的光阱。该平台允许使用红光和远红光时空控制任意 PIF 融合生物分子从各种材料支架中的结合和释放。
