Stoilov L, Mullenders L H, Natarajan A T
Mutat Res. 1986 Aug;174(4):295-301. doi: 10.1016/0165-7992(86)90050-3.
Different levels of replacement of thymidine by 5-bromodeoxyuridine in mammalian DNA have been used to analyze restriction endonuclease-dependent induction of sister-chromatid exchanges and chromosomal aberrations. Data regarding enzyme action in whole cells and in isolated nuclei are presented and discussed. The results indicate a lack of correlation between enzyme effectiveness and the degree of 5-bromodeoxyuridine substitution in the target sequences, specific to the tested restriction endonucleases.
通过5-溴脱氧尿苷对哺乳动物DNA中胸腺嘧啶核苷进行不同程度的取代，已被用于分析限制性内切酶依赖性诱导的姐妹染色单体交换和染色体畸变。本文展示并讨论了关于该酶在全细胞和分离细胞核中作用的数据。结果表明，对于所测试的限制性内切酶而言，酶的有效性与靶序列中5-溴脱氧尿苷的取代程度之间缺乏相关性。
