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限制性内切核酸酶诱导姐妹染色单体交换

Induction of sister-chromatid exchanges by restriction endonucleases.

作者信息

Natarajan A T, Mullenders L H, Meijers M, Mukherjee U

出版信息

Mutat Res. 1985 Sep;144(1):33-9. doi: 10.1016/0165-7992(85)90121-6.

DOI:10.1016/0165-7992(85)90121-6
PMID:2993874
Abstract

Restriction endonucleases Cfo 1, Pvu II, Sma I, Hpa II, Taq I and Hae III were tested for their ability to induce SCEs in CHO cells. The results indicate that the DNA double-strand breaks induced during S-phase by these enzymes lead to an increase in the frequencies of SCEs.

摘要

对限制性内切酶Cfo 1、Pvu II、Sma I、Hpa II、Taq I和Hae III诱导中国仓鼠卵巢(CHO)细胞姐妹染色单体交换(SCE)的能力进行了测试。结果表明,这些酶在S期诱导的DNA双链断裂导致SCE频率增加。

相似文献

1
Induction of sister-chromatid exchanges by restriction endonucleases.限制性内切核酸酶诱导姐妹染色单体交换
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2
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Restriction endonucleases do induce sister-chromatid exchanges in Chinese hamster ovary cells.限制性内切核酸酶确实会诱导中国仓鼠卵巢细胞发生姐妹染色单体交换。
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Influence of bromodeoxyuridine substitution of thymidine on sister-chromatid exchanges and chromosomal aberrations induced by restriction endonucleases.胸腺嘧啶核苷被溴脱氧尿苷取代对限制性内切酶诱导的姐妹染色单体交换和染色体畸变的影响。
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Restriction endonucleases do not induce sister-chromatid exchanges in Chinese hamster ovary cells.限制性内切核酸酶不会在中国仓鼠卵巢细胞中诱导姐妹染色单体交换。
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Influence of dietary carrot on cytostatic drug activity of cyclophosphamide and its main directly acting metabolite: induction of sister-chromatid exchanges in normal human lymphocytes, Chinese hamster ovary cells, and their DNA repair-deficient cell lines.膳食胡萝卜对环磷酰胺及其主要直接作用代谢物的细胞抑制药物活性的影响:在正常人淋巴细胞、中国仓鼠卵巢细胞及其DNA修复缺陷细胞系中诱导姐妹染色单体交换。
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Cytological characterization of Chinese hamster ovary X-ray-sensitive mutant cells, xrs 5 and xrs 6. II. Induction of sister-chromatid exchanges and chromosomal aberrations by X-rays and UV-irradiation and their modulation by inhibitors of poly(ADP-ribose) synthetase and alpha-polymerase.中国仓鼠卵巢X射线敏感突变细胞xrs 5和xrs 6的细胞学特征。II. X射线和紫外线照射诱导的姐妹染色单体交换和染色体畸变及其受聚(ADP - 核糖)合成酶和α - 聚合酶抑制剂的调节
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Molecular mechanisms involved in the production of chromosomal aberrations. III. Restriction endonucleases.染色体畸变产生所涉及的分子机制。III. 限制性内切酶。
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The chromosome-breaking activity of the restriction endonuclease Alu I in CHO cells is independent of the S-phase of the cell cycle.限制性内切酶Alu I在CHO细胞中的染色体断裂活性与细胞周期的S期无关。
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引用本文的文献

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DNA breaks and chromosomal aberrations arise when replication meets base excision repair.当复制遇到碱基切除修复时,就会产生 DNA 断裂和染色体畸变。
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Repair of Site-Specific DNA Double-Strand Breaks in Barley Occurs via Diverse Pathways Primarily Involving the Sister Chromatid.大麦中位点特异性DNA双链断裂的修复主要通过涉及姐妹染色单体的多种途径发生。
Plant Cell. 2014 May;26(5):2156-2167. doi: 10.1105/tpc.114.126607. Epub 2014 May 29.
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X-irradiation of G1 CHO cells induces SCE which are both true and false in BrdU-substituted cells but only false in biotin-dUTP-substituted cells.
对处于G1期的中国仓鼠卵巢细胞进行X射线照射会诱导姐妹染色单体交换,在掺入5-溴脱氧尿苷的细胞中,这种交换既有真性的也有假性的,但在掺入生物素脱氧三磷酸尿苷的细胞中只有假性的。
Chromosome Res. 1999;7(4):277-88. doi: 10.1023/a:1009226930759.
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Double-strand break-induced recombination between ectopic homologous sequences in somatic plant cells.体细胞植物细胞中异位同源序列之间的双链断裂诱导重组。
Genetics. 1999 Jul;152(3):1173-81. doi: 10.1093/genetics/152.3.1173.
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On the importance of DNA strand breaks as the first event to initiate sister chromatid exchange (SCE): experiments with restriction endonuclease BglI.论DNA链断裂作为引发姐妹染色单体交换(SCE)的首个事件的重要性:使用限制性内切酶BglI的实验
Chromosome Res. 1996 Nov;4(7):540-4. doi: 10.1007/BF02261782.
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The diplochromosome of endoreduplicated cells: a new approach to highlight the mechanism of sister chromatid exchange.核内复制细胞的双染色体:一种突出姐妹染色单体交换机制的新方法。
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