Williams J A, Gryson K A, McChesney D J
Peptides. 1986 Mar-Apr;7(2):293-6. doi: 10.1016/0196-9781(86)90228-7.
The binding of 125I-CCK-33 to its receptors prepared from cerebral cortex and cerebellum was studied in four species: mouse, rat, hamster, and guinea pig. Only the guinea pig showed significant binding to membranes from cerebellum and this binding was comparable to that observed for cerebral cortex. In all four species, the order of potency of unlabeled analogs to compete for the binding site was CCK-8 greater than CCK-33 greater than desulfated CCK-8 greater than CCK-4. While the affinity for CCK-8 and CCK-33 was similar in the various species, the relative affinity for desulfated CCK-8 and CCK-4 was less for hamster and guinea pig, indicating species differences in receptor specificity, as well as in regional localization.
研究了125I-CCK-33与从小鼠、大鼠、仓鼠和豚鼠这四种动物的大脑皮层和小脑中制备的受体的结合情况。只有豚鼠的小脑膜显示出显著的结合，且这种结合与大脑皮层中观察到的结合相当。在所有这四个物种中，未标记类似物竞争结合位点的效力顺序为：CCK-8大于CCK-33大于去硫酸化CCK-8大于CCK-4。虽然CCK-8和CCK-33在不同物种中的亲和力相似，但仓鼠和豚鼠对去硫酸化CCK-8和CCK-4的相对亲和力较低，这表明在受体特异性以及区域定位方面存在物种差异。
