Fukumoto H, Seino S, Imura H, Seino Y, Eddy R L, Fukushima Y, Byers M G, Shows T B, Bell G I
Howard Hughes Medical Institute, Chicago, IL.
Proc Natl Acad Sci U S A. 1988 Aug;85(15):5434-8. doi: 10.1073/pnas.85.15.5434.
cDNA clones encoding a glucose transporter-like protein have been isolated from adult human liver and kidney cDNA libraries by cross-hybridization with the human HepG2/erythrocyte glucose transporter cDNA. Analysis of the sequence of this 524-amino acid glucose transporter-like protein indicates that it has 55.5% identity with the HepG2/erythrocyte glucose transporter as well as a similar structural organization. Studies of the tissue distribution of the mRNA coding for this glucose transporter-like protein in adult human tissues indicate that the highest amounts are present in liver with lower amounts in kidney and small intestine. The amounts of glucose transporter-like mRNA in other tissues, including colon, stomach, cerebrum, skeletal muscle, and adipose tissue, were below the level of sensitivity of our assay. The single-copy gene encoding this glucose transporter-like protein has been localized to the q26.1----q26.3 region of chromosome 3.
通过与人肝癌细胞系HepG2/红细胞葡萄糖转运体cDNA进行交叉杂交，已从成人肝脏和肾脏cDNA文库中分离出编码一种葡萄糖转运体样蛋白的cDNA克隆。对这种由524个氨基酸组成的葡萄糖转运体样蛋白的序列分析表明，它与HepG2/红细胞葡萄糖转运体有55.5%的同源性，且结构组织相似。对编码该葡萄糖转运体样蛋白的mRNA在成人组织中的分布研究表明，肝脏中的含量最高，肾脏和小肠中的含量较低。在其他组织中，包括结肠、胃、大脑、骨骼肌和脂肪组织，葡萄糖转运体样mRNA的含量低于我们检测方法的灵敏度水平。编码这种葡萄糖转运体样蛋白的单拷贝基因已定位到3号染色体的q26.1----q26.3区域。
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