Soboleva Tatiana, Benninghoff Abby D, Berreau Lisa M
Department of Chemistry & Biochemistry, Utah State University, Logan, UT 84322-0300.
Department of Animal, Dairy and Veterinary Sciences, Utah State University, Logan, UT 84322-4815.
Chempluschem. 2017 Dec;82(12):1408-1412. doi: 10.1002/cplu.201700524. Epub 2017 Dec 13.
Signaling molecules, including HS and CO, are involved in a complex interplay within cells to maintain cellular homeostasis. In order to investigate the intracellular interplay of different gasotransmitters, new molecular tools are needed that combine sensing and release capabilities of different small molecules in a single, multifunctional, and highly-regulated molecular platform. This report gives the first example of a combined HS sensor/CO-releasing molecule. This flavonol-based molecular tool operates intracellularly via a highly regulated AND logic gated input-output sequence and provides fluorescent feedback for the HS detection and CO release steps. This linear sequence can be combined with a fluorescent molecular sensor for CO detection via a third distinct emission. Overall, this is the first molecular framework that can combine the sensing of HS with the controlled release of CO, two gaseous molecules that are known to exhibit reciprocal regulation and have overlapping targets in cellular environments.
包括硫化氢（HS）和一氧化碳（CO）在内的信号分子参与细胞内复杂的相互作用以维持细胞内稳态。为了研究不同气体递质在细胞内的相互作用，需要新的分子工具，这些工具能在单一、多功能且高度调控的分子平台中结合不同小分子的传感和释放能力。本报告给出了首个硫化氢传感器/一氧化碳释放分子组合的示例。这种基于黄酮醇的分子工具通过高度调控的“与”逻辑门控输入-输出序列在细胞内发挥作用，并为硫化氢检测和一氧化碳释放步骤提供荧光反馈。这个线性序列可以与用于检测一氧化碳的荧光分子传感器相结合，通过第三种不同的发射实现检测。总体而言，这是首个能够将硫化氢传感与一氧化碳的可控释放相结合的分子框架，这两种气体分子在细胞环境中已知会表现出相互调节且具有重叠的靶点。
