缺水及遗传性尿崩症大鼠穹窿下器官中心房钠尿肽（6-33）结合位点增加。

Increased atrial natriuretic peptide (6-33) binding sites in the subfornical organ of water deprived and Brattleboro rats.

作者信息

Saavedra J M, Israel A, Correa F M, Kurihara M

出版信息

Proc Soc Exp Biol Med. 1986 Sep;182(4):559-63. doi: 10.3181/00379727-182-4-rc1.

Abstract

Binding sites for rat atrial natriuretic peptide (6-33) (ANP) were quantitated in the subfornical organ of chronically dehydrated homozygous Brattleboro rats unable to synthesize vasopressin; heterozygous Brattleboro rats, their controls, Long Evans rats and Long Evans rats after 4 days of water deprivation. Brain sections were incubated in the presence of 125I-ANP and the results analyzed by autoradiography coupled to computerized microdensitometry and comparison to 125I-standards. Brattleboro rats and water deprived Long Evans rats presented a higher number of ANP binding sites than their normally hydrated controls. Our results suggest a role of ANP binding sites in the subfornical organ in the central regulation of fluid balance and vasopressin secretion.

摘要

在无法合成血管加压素的慢性脱水纯合布拉特洛伯大鼠的穹窿下器官中，对大鼠心房利钠肽（6 - 33）（ANP）的结合位点进行了定量分析；杂合布拉特洛伯大鼠及其对照、长 Evans 大鼠以及水剥夺 4 天后的长 Evans 大鼠。将脑切片在 125I - ANP 存在的情况下孵育，通过放射自显影结合计算机微密度测定法分析结果，并与 125I 标准品进行比较。布拉特洛伯大鼠和水剥夺的长 Evans 大鼠的 ANP 结合位点数量高于正常水合状态的对照。我们的结果表明，穹窿下器官中的 ANP 结合位点在液体平衡和血管加压素分泌的中枢调节中发挥作用。