Fujita Ryosuke, Kato Fumihiro, Kobayashi Daisuke, Murota Katsunori, Takasaki Tomohiko, Tajima Shigeru, Lim Chang-Kweng, Saijo Masayuki, Isawa Haruhiko, Sawabe Kyoko
Department of Medical Entomology, National Institute of Infectious Diseases, Tokyo, Japan.
Isotope Imaging Laboratory, Creative Research Institution, Hokkaido University, Sapporo, Japan.
Heliyon. 2018 Aug 23;4(8):e00736. doi: 10.1016/j.heliyon.2018.e00736. eCollection 2018 Aug.
In the growth kinetics analysis of flaviviruses in C6/36 cell lines obtained from the Japanese Collection of Research Bioresources (JCRB) Cell Bank and the European Collection of Authenticated Cell Culture (ECACC), these two cells line showed different viral susceptibility for Zika virus (ZIKV), Dengue virus (DENV), and Japanese encephalitis virus (JEV). Next-generation sequencing (NGS) analysis revealed that the C6/36 JCRB strain was persistently infected with two viruses without showing any cytopathic effects. The complete sequence analysis demonstrated that the one virus was Menghai rhabdovirus (MERV), which has been found from mosquito. The other virus was a novel virus, designated as Shinobi tetravirus (SHTV). Interestingly, the viral susceptibility of these two strains was almost even for Sindbis virus and Getah virus. We cloned SHTV and MERV from JCRB C6/36 cell line and then re-infected them into another C6/36 cell line, resulting in the reproduction of persistent infection with each virus. ZIKV growth was suppressed in SHTV and/or MERV re-infected C6/36 cells also. To our knowledge, this is the first demonstration that persistent infection with rhabdovirus and/or permutotetravirus suppressed flavivirus replication in mosquito cells.
在对从日本生物资源研究保存库（JCRB）细胞库和欧洲认证细胞培养物保藏中心（ECACC）获得的C6/36细胞系中的黄病毒进行生长动力学分析时，这两种细胞系对寨卡病毒（ZIKV）、登革病毒（DENV）和日本脑炎病毒（JEV）表现出不同的病毒易感性。下一代测序（NGS）分析显示，C6/36 JCRB株持续感染两种病毒，且未表现出任何细胞病变效应。完整序列分析表明，其中一种病毒是从蚊子中发现的勐海弹状病毒（MERV）。另一种病毒是一种新型病毒，命名为忍术四病毒（SHTV）。有趣的是，这两种毒株对辛德毕斯病毒和盖塔病毒的病毒易感性几乎相同。我们从JCRB C6/36细胞系中克隆了SHTV和MERV，然后将它们重新感染到另一个C6/36细胞系中，导致每种病毒都出现持续感染的再现。在SHTV和/或MERV重新感染的C6/36细胞中，ZIKV的生长也受到抑制。据我们所知，这是首次证明弹状病毒和/或排列四病毒的持续感染会抑制蚊子细胞中的黄病毒复制。
