Department of Chemical Engineering, Federal University of São Carlos, Rod. Washington Luis, km 235, São Carlos, SP, CEP 13565-905, Brazil.
Bioprocess Biosyst Eng. 2018 Dec;41(12):1807-1816. doi: 10.1007/s00449-018-2003-3. Epub 2018 Aug 30.
The production of 1,3-propanediol from crude glycerol and mixed anaerobic sludge was investigated in batch experiments and continuous reactors. Using a 2 complete factorial design, the effects of the concentration of glycerol (22-30 g L), KHPO (1.50-2.00 g L), and vitamin B12 (7-8 mg L) were examined in batch reactors. As an evaluated response, the highest 1,3-PD yields occurred for high concentrations of vitamin B12 and low levels of KHPO, reaching 0.57 g g glycerol consumed. The variable glycerol concentration was not significant in the studied range. In addition, the condition that provided the best 1,3-PD yield was applied to an anaerobic fluidized bed reactor fed with crude glycerol (26.0 g L), which was monitored as the hydraulic retention time (HRT) decreased from 36 to 12 h. The greatest 1,3-PD yield, of 0.31 g g glycerol, was obtained with an HRT of 28 h.
从粗甘油和混合厌氧污泥中生产 1,3-丙二醇的研究在分批实验和连续反应器中进行。使用 2 完整因子设计，考察了甘油浓度（22-30 g/L）、KHPO（1.50-2.00 g/L）和维生素 B12（7-8 mg/L）在分批式反应器中的影响。作为评估的响应，维生素 B12 浓度高、KHPO 浓度低时，1,3-PD 的最高产率为 0.57 g/g 甘油消耗。在所研究的范围内，甘油浓度变量并不显著。此外，在以 26.0 g/L 粗甘油为进料的厌氧流化床反应器中应用了提供最佳 1,3-PD 产率的条件，随着水力停留时间（HRT）从 36 小时降至 12 小时进行监测。在 HRT 为 28 小时时，获得了最大的 1,3-PD 产率，为 0.31 g/g 甘油。
