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单细胞蓝藻聚球藻属6803菌株中氨肽酶P的特性研究

Characterization of Aminopeptidase P from the Unicellular Cyanobacterium Synechocystis sp. PCC6803.

作者信息

Baik A S, Mironov K S, Arkhipov D V, Piotrovskii M S, Pojidaeva E S

机构信息

Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Moscow, 127276, Russia.

出版信息

Dokl Biochem Biophys. 2018 Jul;481(1):190-194. doi: 10.1134/S1607672918040038. Epub 2018 Aug 31.

DOI:10.1134/S1607672918040038
PMID:30168056
Abstract

The PepP protein has been purified in vitro and characterized for the first time. It is encoded by the sll0136 gene of the unicellular cyanobacterium Synechocystis sp. PCC6803. It is established that the PepP protein is a Mn-dependent Xaa-Pro-specific aminopeptidase. The protein in the reaction of hydrolysis of the fluorescent peptide Lys(N-Abz)-Pro-Pro-pNA has a maximal activity at pH 7.6 and 32°C.

摘要

PepP蛋白已在体外得到纯化并首次进行了特性鉴定。它由单细胞蓝藻集胞藻PCC6803的sll0136基因编码。现已确定PepP蛋白是一种依赖锰的Xaa-Pro特异性氨肽酶。该蛋白在水解荧光肽Lys(N-Abz)-Pro-Pro-pNA的反应中，于pH 7.6和32°C时具有最大活性。

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