Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, China.
School of Pharmacy, Jiangsu University, Zhenjiang, China.
J Sep Sci. 2018 Nov;41(21):3974-3984. doi: 10.1002/jssc.201800557. Epub 2018 Sep 20.
To determine the presence of multi-class mycotoxins in Menthae haplocalycis, a sample processing procedure based on a modified quick, easy, effective, rugged, and safe method, and a rapid and accurate testing method using ultra-fast performance liquid chromatography coupled with tandem mass spectrometry, was developed and validated. We systematically evaluated the methodology for multi-mycotoxin analysis in the Menthae haplocalycis samples, and chose matrix-matched calibration curves as a reference to calculate the recoveries. Overall, the average recoveries varied between 67.1 and 103%, with relative standard deviations ranging from 0.34 to 10.3%. The optimized and validated method was applied to detect the presence of the target mycotoxins in 40 batches of Menthae haplocalycis samples. Results showed that the levels of mycotoxins varied among the samples. The most prevalent mycotoxin was tentoxin, followed by alternariol, alternariol monomethyl ether, zearalenone, fumonisin B , fumonisin B , ochratoxin A, aflatoxin B , aflatoxin B , aflatoxin G , and T-2 toxin. The analytical method developed herein could be applied for the routine monitoring of multi-mycotoxins in Menthae haplocalycis.
为了确定薄荷单双体中多类霉菌毒素的存在，开发并验证了一种基于改良的快速、简便、有效、坚固、安全方法的样品处理程序，以及一种使用超快速性能液相色谱串联质谱的快速准确检测方法。我们系统地评估了薄荷单双体样品中多霉菌毒素分析的方法学，并选择基质匹配校准曲线作为参考来计算回收率。总的来说，平均回收率在 67.1%至 103%之间，相对标准偏差在 0.34%至 10.3%之间。优化和验证后的方法应用于检测 40 批薄荷单双体样品中目标霉菌毒素的存在。结果表明，霉菌毒素的水平在样品之间有所不同。最常见的霉菌毒素是 tentoxin，其次是 alternariol、alternariol monomethyl ether、zearalenone、fumonisin B₁、fumonisin B₂、ochratoxin A、aflatoxin B₁、aflatoxin B₂、aflatoxin G₁、和 T-2 毒素。本文开发的分析方法可用于薄荷单双体中多霉菌毒素的常规监测。
