Feng Yufei, Jing Zhongxu, Li Yongji, Lv Shaowa, Li Weinan, Cai Guofeng, Yang Danqi, Wang Yanhong
Key Laboratory of Chinese Materia Medica in Ministry of Education, Heilongjiang University of Chinese Medicine, Harbin, China.
The Second Affiliated Hospital, Heilongjiang University of Chinese Medicine, Harbin, China.
Biomed Chromatogr. 2019 Feb;33(2):e4376. doi: 10.1002/bmc.4376. Epub 2018 Nov 6.
Shuang Huang Lian Injection (SHLI) has been used in China for over 30 years as an effective and widely used Chinese herbal prescription to treat acute respiratory infectious. SHLI has, however, caused many severe anaphylactoid reactions. It is important to identify the potential anaphylactoid components of SHLI. Spectrum-effect relationships were used to explore potentially anaphylactoid components. Based on the original herbal formula, honeysuckle, Fructus Forsythiae and Radix Scutellariae extracts were prepared and combined in appropriate proportions. The preparations were then injected into the caudal vein of rats to obtain in vivo serum samples for pharmacological evaluation and fingerprint analysis. The release rate of β-hexosaminidase from RBL-2H cells and plasma histamine level was used as the pharmacological index. Chromatographic fingerprint analysis identified 22 common peaks. Regression analysis and correlation analysis were used to calculate the relationships between the peaks and the pharmacological effects and identified peaks 5, 6, 11, 12 and 17 as likely anaphylactoid agents. The correlated peaks were identified by comparing the fingerprints with in vitro samples and reference standard samples and the structure was identified by UPLC-TOF-MS. This study established a prospective method to clarify the anaphylactoid components in SHLI, which would provide guidances for screening anaphylactoid components in other traditional Chinese medicine injections.
双黄连注射液（SHLI）在中国已使用30多年，是一种治疗急性呼吸道感染的有效且广泛应用的中药方剂。然而，SHLI引发了许多严重的类过敏反应。识别SHLI潜在的类过敏成分很重要。采用谱效关系法探索潜在的类过敏成分。基于原草药配方，制备金银花、连翘和黄芩提取物，并按适当比例混合。然后将制剂注入大鼠尾静脉，以获得用于药理评价和指纹图谱分析的体内血清样本。以RBL-2H细胞β-己糖胺酶释放率和血浆组胺水平作为药理指标。色谱指纹图谱分析鉴定出22个共有峰。采用回归分析和相关分析计算峰与药理效应之间的关系，确定峰5、6、11、12和17可能为类过敏剂。通过将指纹图谱与体外样品和参考标准样品进行比较来鉴定相关峰，并通过超高效液相色谱-飞行时间质谱鉴定结构。本研究建立了一种前瞻性方法来阐明SHLI中的类过敏成分，这将为筛选其他中药注射液中的类过敏成分提供指导。
