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基于超高效液相色谱-四极杆飞行时间质谱联用技术分析谱效关系筛选西洋参皂苷抗疲劳成分

Screening anti-fatigue components of American ginseng saponin by analyzing spectrum-effect relationship coupled with UPLC-Q-TOF-MS.

作者信息

Lin Meiyu, Hu Shaiping, Zeng Qi, Xiao Bixia, He Yao

机构信息

School of Pharmacy, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China.

School of Pharmacy, Changsha Medical University, Changsha, Hunan 410209, China.

出版信息

Asian Biomed (Res Rev News). 2023 Oct 18;17(4):163-172. doi: 10.2478/abm-2023-0057. eCollection 2023 Aug.

DOI:10.2478/abm-2023-0057
PMID:37860679
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10584381/
Abstract

BACKGROUND

American ginseng has an obvious anti-fatigue effect, but the effective material basis is still unclear. The spectrum-effect relationship is a scientific method that studies the correlations between chemical spectra and pharmacological effect.

OBJECTIVE

To reveal the real bioactive compounds in American ginseng saponin (AGS) based on a study of the underlying correlations between these compounds' occurrence in rat serum after their intake of AGS and the anti-fatigue effect of AGS.

METHODS

We utilized ultra-performance liquid chromatography (UPLC) with quadrupole and time-of-flight mass spectrometry (Q-TOF-MS) to analyze the extract of AGS and its constituents in serum after oral administration in rats. The anti-fatigue effect of AGS in rats was measured using the time weight-bearing swimming technique, the content of blood urea nitrogen, hepatic glycogen, and blood lactic acid. The relationship between the peak area values in fingerprints from rat serum and pharmacodynamic parameters of AGS was established using correlation analysis with partial least square regression (PLSR) method and gray correlation method.

RESULTS

We detected and identified 22 compounds from extract, and 8 prototype components from serum. Through PLSR and gray correlation method, it was found that the ginsenosides Re, Rb1, and Rb2 were significantly positively related to the pharmacodynamic data.

CONCLUSIONS

Based on the spectrum-effect relationship, PLSR and gray correlation method can be used to screen for the anti-fatigue components available in AGS. Such an approach is of practical significance as it provides an effective means for exploring the material basis for the efficacy of American ginseng, particularly as an anti-fatigue agent.

摘要

背景

西洋参具有明显的抗疲劳作用，但其有效物质基础仍不清楚。谱效关系是研究化学光谱与药理效应之间相关性的科学方法。

目的

通过研究西洋参皂苷（AGS）摄入后大鼠血清中这些化合物的存在情况与AGS抗疲劳作用之间的潜在相关性，揭示AGS中的真正生物活性化合物。

方法

我们利用超高效液相色谱（UPLC）结合四极杆和飞行时间质谱（Q-TOF-MS）分析大鼠口服给药后血清中AGS提取物及其成分。采用负重游泳时间、血尿素氮、肝糖原和血乳酸含量测定AGS对大鼠的抗疲劳作用。采用偏最小二乘回归（PLSR）法和灰色关联法进行相关性分析，建立大鼠血清指纹图谱峰面积值与AGS药效学参数之间的关系。

结果

从提取物中检测并鉴定出22种化合物，从血清中检测出8种原型成分。通过PLSR和灰色关联法发现，人参皂苷Re、Rb1和Rb2与药效学数据呈显著正相关。

结论

基于谱效关系，PLSR和灰色关联法可用于筛选AGS中的抗疲劳成分。这种方法具有实际意义，为探索西洋参功效的物质基础，特别是作为抗疲劳剂的物质基础提供了有效手段。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4903/10584381/32fcf008b867/j_abm-2023-0057_fig_006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4903/10584381/d8f2ff42bfd7/j_abm-2023-0057_fig_001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4903/10584381/36af328e3728/j_abm-2023-0057_fig_002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4903/10584381/8be4efffe018/j_abm-2023-0057_fig_003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4903/10584381/e00325c9a407/j_abm-2023-0057_fig_004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4903/10584381/2ba37af524f5/j_abm-2023-0057_fig_005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4903/10584381/32fcf008b867/j_abm-2023-0057_fig_006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4903/10584381/d8f2ff42bfd7/j_abm-2023-0057_fig_001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4903/10584381/36af328e3728/j_abm-2023-0057_fig_002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4903/10584381/8be4efffe018/j_abm-2023-0057_fig_003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4903/10584381/e00325c9a407/j_abm-2023-0057_fig_004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4903/10584381/2ba37af524f5/j_abm-2023-0057_fig_005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4903/10584381/32fcf008b867/j_abm-2023-0057_fig_006.jpg

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