Rizzo Annalisa, Ceci Edmondo, Guaricci Antonio Ciro, Sciorsci Raffaele Luigi
Department of Veterinary Medicine, University of Bari Aldo Moro, Valenzano (BA), Italy.
Reprod Domest Anim. 2019 Feb;54(2):195-198. doi: 10.1111/rda.13325. Epub 2018 Oct 8.
Recently, a new molecule, kisspeptin (Kp), and in particular Kisspeptin 10 (Kp10), was implicated in stimulating the hypothalamic-pituitary-gonadal axis. The aim of this study was to evaluate circulating Kp10 levels in the early post-partum period of the dairy cow. Blood samples were collected from 40 dairy cows, at 10 (T10), 12 (T12), 14 (T14) and 16 (T16) days after calving. Progesterone (P4) levels were evaluated using ELISA, and levels of oestrogens (E2) and Kp were evaluated using a radio-immunologic method. After an initial plateau, Kp10 significantly increased at T14 and decreased at T16. The P4 and E2 mean serum values remained in the physiological range. It is likely that Kp10 enhanced hypothalamic GnRH release as well as pituitary gonadotropin secretion, thus promoting follicular growth and the increase in E2 levels, which might have further enhanced Kp10 release through a positive feedback loop. To our best knowledge, this is the first report on the range of Kp10 blood concentration during the early post-partum period in the dairy cow. The results of our study will increase our current understanding of the complex neuro-endocrine crosstalk underlying the resumption of ovarian cyclicity in the dairy cow.
最近，一种新分子—— kisspeptin（Kp），尤其是 Kisspeptin 10（Kp10），被认为与刺激下丘脑 - 垂体 - 性腺轴有关。本研究的目的是评估奶牛产后早期循环中的 Kp10 水平。在产犊后第 10 天（T10）、12 天（T12）、14 天（T14）和 16 天（T16）从 40 头奶牛采集血样。使用酶联免疫吸附测定法（ELISA）评估孕酮（P4）水平，使用放射免疫法评估雌激素（E2）和 Kp 水平。在最初的平稳期后，Kp10 在 T14 时显著升高，在 T16 时下降。P4 和 E2 的平均血清值保持在生理范围内。Kp10 可能增强了下丘脑促性腺激素释放激素（GnRH）的释放以及垂体促性腺激素的分泌，从而促进卵泡生长和 E2 水平升高，而 E2 水平升高可能通过正反馈回路进一步增强 Kp10 的释放。据我们所知，这是关于奶牛产后早期 Kp10 血液浓度范围的首次报道。我们的研究结果将增进我们目前对奶牛卵巢周期性恢复背后复杂神经内分泌相互作用的理解。
