[生殖支原体感染对不育男性精液质量的影响]

[Impact of Mycoplasma genitalium infection on the semen quality of infertile males].

作者信息

Yan Ze-Chen, Shang Xue-Jun, Liu Wei, Wan Xiu-Xia, Wan Chang-Chun, Xu Song, Zhong Yong, Weng Zhi-Qiang

机构信息

Department of Surgery, the First Affiliated Hospital, Zhengzhou University, Zhengzhou, Henan 450052, China.

Department of Andrology, Nanjing School of Clinical Medicine, Southern Medical University / Nanjing General Hospital of Nanjing Military Region, Nanjing, Jiangsu 210002, China.

出版信息

Zhonghua Nan Ke Xue. 2018 Apr;24(4):317-321.

PMID:30168950

Abstract

OBJECTIVE

To explore Mycoplasma genitalium (MG) infection in the urogenital tract of infertile men and its influence on semen quality.

METHODS

Semen samples were collected from 352 infertile males in the Center of Reproductive Medicine of Nanjing General Hospital from March to July 2015. MG infection was detected by real-time fluorescence simultaneous amplification and testing and semen analyses were conducted according to the WHO Laboratory Manual for the Examination and Processing of Human Semen (5th Ed) on the semen pH value, semen volume, total sperm count, sperm concentration, total sperm motility, percentages of progressively motile sperm (PMS) and immotile sperm (IMS), and sperm DNA fragmentation index (DFI). The data obtained were subjected to statistical analysis by t-test and non-parametric test (Wilcoxon test).

RESULTS

MG infection was found in 3.4% (12/352) of the infertile patients. Compared with the MG-positive cases, the MG-negative ones showed a significantly higher semen volume (［2.85 ± 0.14］ vs ［3.84 ± 0.12］ ml, P = 0.008) and percentage of PMS (［15.86±1.72］ vs ［60.95 ± 5.63］ %, P = 0.032) but a lower DFI (［30.73 ±2.24］ vs ［20.71 ± 1.55］%, P = 0.014). However, no statistically significant differences were observed between the two groups in the semen pH value (7.38 ±0.02 vs 7.39 ± 0.01, P = 0.774), sperm concentration (［52.96 ± 15.78］ vs ［60.05 ± 4.29］×10⁶/ml, P = 0.683), sperm count (［154.15 ± 46.37］ vs ［221.56 ± 15.43］×106, P = 0.236), total sperm motility (［29.04 ± 3.11］ vs ［33.52 ± 1.51］ %, P = 0.626), or percentage of IMS (［23.57 ± 0.99］ vs ［62.34 ± 1.69］ %, P = 0.691).

CONCLUSIONS

Urogenital MG infection is common in infertile males and potentially affects the semen quality, especially sperm vitality of the patient.

摘要

目的

探讨不育男性泌尿生殖道解脲脲原体（MG）感染情况及其对精液质量的影响。

方法

收集2015年3月至7月在南京军区南京总医院生殖医学中心就诊的352例不育男性的精液样本。采用实时荧光同步扩增检测法检测MG感染，并依据世界卫生组织《人类精液检查与处理实验室手册》（第5版）对精液pH值、精液量、精子总数、精子浓度、精子总活力、前向运动精子（PMS）百分比、不活动精子（IMS）百分比及精子DNA碎片指数（DFI）进行精液分析。所得数据采用t检验和非参数检验（Wilcoxon检验）进行统计分析。

结果

352例不育患者中MG感染率为3.4%（12/352）。与MG阳性病例相比，MG阴性病例的精液量显著更高（［2.85±0.14］ vs ［3.84±0.12］ml，P = 0.008）、PMS百分比显著更高（［15.86±1.72］ vs ［60.95±5.63］%，P = 0.032），但DFI更低（［30.73±2.24］ vs ［20.71±1.55］%，P = 0.014）。然而，两组在精液pH值（7.38±0.02 vs 7.39±0.01，P = 0.774）、精子浓度（［52.96±15.78］ vs ［60.05±4.29］×10⁶/ml，P = 0.683）、精子总数（［154.15±46.37］ vs ［221.56±15.43］×10⁶，P = 0.236）、精子总活力（［29.04±3.11］ vs ［33.52±1.51］%，P = 0.626）或IMS百分比（［23.57±0.99］ vs ［62.34±1.69］%，P = 0.691）方面差异均无统计学意义。