Khursigara Cezar M, Koval Susan F, Moyles Dianne M, Harris Robert J
a Department of Molecular and Cellular Biology, University of Guelph, Guelph, ON N1G 2W1, Canada.
b Molecular and Cellular Imaging Facility, Advanced Analysis Centre, University of Guelph, Guelph, ON N1G 2W1, Canada.
Can J Microbiol. 2018 Sep;64(9):601-617. doi: 10.1139/cjm-2018-0091.
A singular feature of all prokaryotic cells is the presence of a cell envelope composed of a cytoplasmic membrane and a cell wall. The introduction of bacterial cell fractionation techniques in the 1950s and 1960s along with developments in procedures for electron microscopy opened the window towards an understanding of the chemical composition and architecture of the cell envelope. This review traces the contribution of Terry Beveridge in these endeavours, beginning with his doctoral studies in the 1970s on the structure of paracrystalline surface arrays (S-layers), followed by an exploration of cryogenic methods for preserving bacteria for ultrastructural analyses. His insights are reflected in a current example of the contribution of cryo-electron microscopy to S-layer studies - the structure and assembly of the surface array of Caulobacter crescentus. The review then focuses on Terry's contributions to imaging the ultrastructure of bacterial cell envelopes and to the development of cryo-electron microscopy techniques, including the use of CEMOVIS (Cryo-electron Microscopy of Vitreous Sections) to "see" the ultrastructure of the Gram-positive cell envelope - his last scientific endeavour.
所有原核细胞的一个显著特征是存在由细胞质膜和细胞壁组成的细胞包膜。20世纪50年代和60年代细菌细胞分级分离技术的引入，以及电子显微镜技术的发展，为了解细胞包膜的化学成分和结构打开了一扇窗。这篇综述追溯了特里·贝弗里奇在这些努力中的贡献，从他20世纪70年代关于准晶体表面阵列（S层）结构的博士研究开始，接着探讨了用于保存细菌以进行超微结构分析的低温方法。他的见解体现在低温电子显微镜对S层研究贡献的一个当前例子中——新月柄杆菌表面阵列的结构和组装。然后，这篇综述聚焦于特里在对细菌细胞包膜超微结构成像以及低温电子显微镜技术发展方面的贡献，包括使用CEMOVIS（玻璃切片低温电子显微镜）来“观察”革兰氏阳性细胞包膜的超微结构——这是他最后的科学努力。
