利用低拷贝数重复序列在体细胞杂种中绘制人类染色体图谱。

Mapping human chromosomes in somatic cell hybrids using a low-copy-number repetitive sequence.

作者信息

Shaw D J, Brook J D, Brown C S, Thomas N S

出版信息

Somat Cell Mol Genet. 1986 Jul;12(4):333-7. doi: 10.1007/BF01570727.

Abstract

We have isolated a human-specific repetitive sequence with a copy number of several hundred (p11L26). Using Southern blots of EcoR1-digested DNA from somatic cell hybrids containing one or a few human chromosomes, band patterns specific for those chromosomes can be generated by hybridization with p11L26. Genomic copies of the sequence have also been mapped to subchromosomal regions using translocations. The probe offers a useful addition to the standard techniques for mapping human chromosomes in hybrid cell lines.

摘要

我们已经分离出一种人类特异性重复序列，其拷贝数为几百个（p11L26）。使用来自含有一条或几条人类染色体的体细胞杂种经EcoR1消化的DNA进行Southern印迹分析，通过与p11L26杂交可以产生那些染色体特有的条带模式。该序列的基因组拷贝也已通过易位定位到亚染色体区域。该探针为在杂交细胞系中绘制人类染色体的标准技术提供了有用的补充。