人类常染色体磷酸甘油酸激酶序列定位于19号染色体。

Mapping of human autosomal phosphoglycerate kinase sequence to chromosome 19.

作者信息

Gartler S M, Riley D E, Lebo R V, Cheung M C, Eddy R L, Shows T B

出版信息

Somat Cell Mol Genet. 1986 Jul;12(4):395-401. doi: 10.1007/BF01570734.

Abstract

In order to map human PGK sequences, DNA was prepared from 55 human-mouse somatic cell lines. The DNA was digested to completion with HindIII and Southern filters prepared. These filters were hybridized at high stringency conditions to a human PGK cDNA. Mouse and human X-linked and autosomal bands were distinguished and, in addition to known X-linked sequences, two autosomal PGK sequences were mapped: a 1-kb band to chromosome 19 and a 5-kb band to chromosome 6. The PGK cDNA probe was also hybridized to flow-sorted chromosomes confirming the presence of PGK sequences on the X chromosome and chromosomes 6 and 19.

摘要

为了绘制人类磷酸甘油酸激酶（PGK）序列图谱，从55个人-鼠体细胞系中制备了DNA。用 HindIII 将DNA完全消化，并制备了Southern杂交滤膜。这些滤膜在高严格条件下与人类PGK cDNA杂交。区分了小鼠和人类的X连锁和常染色体条带，除了已知的X连锁序列外，还绘制了两个常染色体PGK序列：一条1 kb的条带位于19号染色体，一条5 kb的条带位于6号染色体。PGK cDNA探针也与流式细胞仪分选的染色体杂交，证实了X染色体以及6号和19号染色体上存在PGK序列。